摘要
目的探讨参麦注射液在单侧输尿管梗阻(UUO)大鼠肾间质纤维化进程中的可能作用。方法成年SD大鼠54只,随机分为假手术组、模型组和参麦注射液治疗组。假手术组仅完成开腹过程,不结扎输尿管;模型组和参麦注射液治疗组行开腹左侧输尿管结扎术,术后参麦治疗组每天腹腔注射参麦注射液3 mL/(kg.d),假手术组与模型组则每天腹腔注射等量生理盐水。分别于实验的第7、14、21天各组处死动物6只,取左肾组织进行HE染色和α-平滑肌肌动蛋白(α-SMA)免疫组化检查,并测定梗阻侧肾组织中SOD和MDA含量。结果与模型组比较,参麦注射液治疗组肾小管间质病理改变明显减轻,肾间质α-SMA表达减少;肾组织中SOD活性增加,MDA含量下降。结论参麦注射液可通过减少氧化应激,减少肾间质α-SMA表达而抑制肾间质纤维化进程。
Objective To investigate the effect of Shenmai on renal interstitial fibrosis in rats with due to unilateral ureteral obstruction（UUO）.Methods 54 SD rats were randomly divided into sham-operation group,UUO group and Shenmai group.Rats in UUO group and Shenmai group underwent left unilateral ureteral ligation.Rats in sham-operation group had their ureters manipulated but not ligated.Shenmai injection was injected intraperitoneally（3 mL/kg·d） in Shengmai group.Meantime,same volume of saline was given in sham-operation group and UUO groups as Shengmai group.The rats were sacrificed on the 7th,14th and 21st day.Renal tissue superoxide dismutase（SOD） and malondialdehyde（MDA） content were detect and renal tissue α-smooth muscle actin（α-SMA） were assessed by immunohistochemistry study.Results The tubulointerstitial pathological changes of Shenmai group was better than that of UUO group.Compared with UUO group,Shenmai group showed a significant increase in SOD and a marked decrease in MDA.Conclusion Shenmai injection can reduce renal oxidative stress injury induced by UUO,reduce the expression of α-SMA in renal interstitial fibrosis.
出处
《四川动物》
CSCD
北大核心
2011年第4期620-623,共4页
Sichuan Journal of Zoology
