摘要
目的研究抗癌药物羟基喜树碱(HCPT)对PC12细胞的毒副作用,探讨HCPT对神经系统的副作用。方法用CCK-8试剂盒(cell counting kit-8)测定不同浓度的HCPT对PC12细胞的生长抑制作用;吖啶橙/溴化乙锭(AO/EB)染色观察细胞生长情况;琼脂糖凝胶电泳验证细胞凋亡的特征性DNA条带;流式细胞术测定一定浓度下不同作用时间的HCPT对细胞凋亡和细胞周期的影响。结果 CCK-8结果表明不同浓度的HCPT对PC12细胞的生长抑制作用不同;琼脂糖凝胶电泳可见凋亡细胞DNA的梯状条带;荧光显微镜下观察到经AO/EB染色的典型凋亡细胞;在相同浓度下,随作用时间的延长细胞的凋亡率也逐渐增加。结论抗癌药物羟基喜树碱对PC12细胞有毒副作用,HCPT对神经系统的副作用与诱导神经细胞凋亡有关,其细胞凋亡与细胞周期分布有关。
Objective To study side effects of the anticancer drug hydroxycamptothecin on PC12 cell and discuss the side effect of HCPT on nervous system.Methods The growth inhibition was studied by CCK-8 assay.The morphologic changes of the apoptotic cells were observed by acridine orange/ethidium bromide dying.DNA of the apoptotic cells was analyzed with agarose gel electrophoresis（AGE）.The apoptosis rates and the cell cycle distribution were studied by flow cytometry after treatment with HCPT.Results CCK-8 assay showed that various concentration of HCPT could effectively inhibit the growth of cell line PC12.DNA ladder was observed in ultraviolet light.The typical apoptotic cells under the fluorescence microscope showed budding phenomena and apoptotic bodies.According to the results of flow cytometry,apoptosis rates increased with the treatment time prolonged under the same HCPT concentration.Conclusion HCPT has side effects and toxic action on PC12 cell.The effect is maybe related with its role of inducing cell apoptosis which might be correlated with distribution of the cell cycle.
出处
《成都医学院学报》
CAS
2011年第2期139-143,共5页
Journal of Chengdu Medical College
基金
成都医学院大学生创新性实验
