摘要
目的探讨肿瘤坏死因子相关凋亡导配体(TRAIL)受体DR4、DR5、DcR1在结肠癌中的表达及临床意义,为TRAIL的抗肿瘤作用提供实验依据。方法采用免疫组化SP法检测35例结肠癌和14例正常结肠黏膜组织中DR4、DR5、DcR1表达水平。结果 35例结肠癌组织中DR5的阳性表达率为27/35(77.14%),DR4的阳性表达率为19/35(54.28%),均明显高于正常结肠黏膜组织4/14(28.57%),差异有统计学意义(P<0.05);DR5的阳性表达率明显高于DR4(P<0.05)。正常结肠黏膜组织中DcR1的阳性表达率为100%,明显高于结肠癌13/35(37.14%),差异有统计学意义(P<0.05)。结论结肠癌中DR4、DR5的高表达使TRAIL用于结肠癌的治疗在理论上具有可行性,DR5可能在TRAIL诱导的凋亡通路中发挥更重要的作用,结肠癌中DcR1的表达可能导致TRAIL诱导的凋亡耐受。
Objective To investigate the expression and significance of TRAIL（TNF-related apoptosis inducing ligand）,DR4,DR5,DcR1 in colorectal carcinoma.Methods The immunohistochemical technique（SP） was used to detect the expression of TRAIL-R,DR4,DR5,DcR1 in 35 cases of colorectal carcinoma and 14 cases of normal colorectal mucosa.Results The positive expression rate of DR5 in colorectal carcinoma was 27/35（77.14%）,DR4 was 19/35（54.28%）,and the positive expression rate of DR5 in colorectal carcinoma was significantly higher than that [4/14（28.57%）] in normal colorectal mucosa（P0.05）,so was the positive expression rate of DR4（P0.05）.The expression of DcR1 in normal colorectal mucosa was 100%,which was significantly higher than that [13/35（37.14%）] in colorectal carcinoma（P0.05）.Conclusion The high expression of DR4,DR5 in colorectal carcinoma provides theoretical feasibility for TRAIL to treat colorectal carcinoma.The DR5 may play an important role in the apoptosis pathway induced by TRAIL.The expression of DcR1 in colorectal carcinoma may result in the tolerance of cell apoptosis induced by TRAIL.
出处
《河北医药》
CAS
2011年第14期2090-2091,共2页
Hebei Medical Journal
