摘要
本实验以皮燕麦２０７９ 成熟种子胚为外植体，其愈伤组织的诱导率最高，叶鞘愈伤组织诱导率为５ ％ ，叶片几乎不发生脱分化。在基本培养基比较中以改良Ｎ６ 培养基诱导效果为佳，愈伤组织出愈率在１－３ｍｇ／Ｌ，２ ，４—Ｄ浓度范围逐渐提高，继续增大时则出愈率降低。胚性愈伤组织形成率受脱分化培养基中２，４ —Ｄ浓度的影响。扫描电镜观察结果显示非胚性与胚性愈伤组织表面结构差异明显；组织切片表明形成的再生植株有胚胎发生与器官发生两种途径，形态上有区别。
The efficiency of callus induction was much higher,if mature embryos of oats were used as explants.No dedifferentiation was observed by using leaf blade as explants.The best medium was modified N 6.The efficiency of callus raise when 2,4-D concentration increased from 1 to 3 mg/L.Overhigh concentration of 2,4_D was worse.The concentration of 2,4-D in dedifferentiation medium had relation to embryogenie callus formation.It was different bettew the surface structure of the embryogenic and non-embryogenic calluses by means of observation by scanning electron microscope.The callus sections showed That the Plantlet regeneration was differentiated ways.
出处
《青海师范大学学报（自然科学版）》
1999年第3期40-45,45,共6页
Journal of Qinghai Normal University(Natural Science Edition)
关键词
皮燕麦
组织培养
胚性愈伤组织
再生苗
植株再生
Oats (Avena Sativa)
Tissue culture
Embryogenic callus
Plantlet regeneration
