摘要
目的研究色素框同源蛋白7（cbx7）基因在结肠癌、胃癌和肝癌组织中的表达变化，探讨这种表达变化与肿瘤发生和发展的相关性。方法收集22例结肠癌患者、20例胃癌患者、30例肝癌患者的肿瘤组织及其切缘组织标本、临床病理资料和随访资料。提取各标本RNA，用荧光定量PCR检测cbx7mRNA含量，分析肿瘤组织中cbx7mRNA表达水平与患者临床特征相关性及与患者术后生存时间的关系。结果结肠癌、胃癌和肝癌组织中cbx7mRNA的相对拷贝数分别为0．010±0．015、0．197±0．195、0．008±0．008；对应各癌组织的切缘正常组织中cbx7mRNA的相对拷贝数分别为0．053±0．042、1．891±1．254、0．030±0．021。与切缘正常组织相比，3种癌组织中的cbx7mRNA含量均明显下调（结肠癌：t=-7．351，P〈0．01；胃癌：t=-5．417，P〈0．01；肝癌：t=-6．680，P〈0．01）。结肠癌患者中，年龄〉55岁组cbx7mRNA相对拷贝数为0．007±0．015，≤55岁组为0．017±0．012，两组间差异有统计学意义（t=-2．586，P=0．022）；有脉管癌栓组cbx7mRNA的相对拷贝数为0．022±0．021，无癌栓组为0．006±0．011，两组间差异有统计学意义（t=-3．175，P=0．010）。通过受试者特征曲线[ROC曲线，曲线下面积为0．769（P=0．033）]确定结肠癌患者cbx7mRNA相对拷贝数的Cut—off值为0．002，小于0．002者确定为表达降低；cbx7低表达的结肠癌患者术后生存时间短，5年生存率仅为30．8％（4／13），而cbx7高表达患者5年生存率达77．8％（7／9），差异有统计学意义（X^2=4．329，P=0．037）。在胃癌和肝癌患者中未见此现象。结论在结肠癌、胃癌、肝癌组织中cbx7的表达明显下降；cbx7mRNA的表达含量变化影响结肠癌患者预后。
Objective To investigate the relationship between ehromobox protein homolog 7 （cbx7） expression and the occurrence and development of eolorectal carcinoma （CRC）, gastric carcinoma （GC） and hepatoeareinoma （HCC） tissues. Methods The samples of neoplastic tissues and the corresponding eutting-edge normal tissues from 22 eases of CRC, 20 eases of GC, 30 cases of HCC were surgically eollected. Level of ebx7 mRNA was detected with a fluorescent quantitative RT-PCR assay, and the eorrelationship among expression of cbx7 mRNA, the patients＇ clinicopathologic features and the surviving time after surgery was analyzed. Results The relative copy number of cbx7 mRNA in carcinomas and the normal tissues was 0. 010 ±0. 015 vs 0. 053 ±0. 042 for CRCs,0. 197 ±0. 195 vs 1. 891 ± 1. 254 for GCs,and 0. 008±0. 008 vs 0. 030 ± 0. 021 for HCCs, respectively. Compared with the corresponding normal tissues, cbx7 expression was significantly downregulated in CRCs, GCs, and HCCs （t = -7. 351, -5. 417 and -6. 680, respectively ,P 〈 0. 01 ）. The expression of cbx7 mRNA in CRCs had significant differences not only between two age groups （the relative copy number of ebx7 mRNA in age 〉 55 group was 0. 007 ± 0. 015, but 0. 017 ±0. 012 in age≤55 group ,t = -2. 586 ,P =0. 022） ; but also between vascular embolus-positive and negative groups （ the level of cbx7 mRNA in positive and negative group was 0. 022 ± 0. 021 vs 0. 006 ± 0. 011 ,t = -3. 175 ,P =0. 010）. The area under the receiver operating characteristics（ROC） curve is 0. 769 （ P = 0. 033）. when the Cut-off value of the relative copy number of cbx7 mRNA was 0. 002 in CRCs. The values less-than 0. 002 were defined as low expression. The CRC patients with low expression of cbx7 had a shorter overall survival time; whose 5 years survival rate was only 30. 8% （4/13）; while the rate was 77.8% （7/9） in high expression of cbx7 group. The difference had statistical significance （X^2 =4. 329,P = 0. 037 ） . The similar differences could not be found among GC and HCC . patients. Conclusion Downregulation of cbx7 expression was very common among multiple carcinomas cases, and the downregulation influenced the prognosis of CRC patients.
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
2011年第7期597-600,共4页
Chinese Journal of Preventive Medicine
基金
北京市自然科学基金（7112024）
高等学校博士学科点专项科研基金（20100001110098）
关键词
肿瘤
基因表达
聚合酶链反应
Neoplasms
Gene expression
Polymerase chain reaction
