摘要
目的 研究雌、孕激素对卵巢癌耐药细胞系ＯＶＣＡＲ－３生长及对阿霉素（ＡＤＭ）耐药的影响。方法 用四甲基偶氮唑蓝（ＭＴＴ）快速比色法，计算细胞生存率，以比较不同浓度雌、孕激素对细胞生长的影响，及雌，孕激素与异搏定对卵巢癌耐药细胞系ＯＶＣＡＲ－３耐药性的影响。
Objective To study the effects of estradiol (E2) and medroxyprogesterone acetate (MPA) on the growth of drug resistant human epithelial ovarian cancer cell line. Methods OVCAR-3 cells, treated by different concentrations of E2, MPA and verapamil, were examined for their growth inhiitions with methyl thiazlyl tetrazolium (MTT, a tetrazolium dye) rapid photocolorimetric assay. Results OVCAR-3 cell growth was inhibted by E2 at high concentrations (10.00- 100.00 μmol/L)and by MPA at 100.00μmol/L, while lower concentrations of E2(0.01-1 .00 μmol/L) and MPA(0.01-10.00 μmol/L) had no effect on OVCAR-3 cell growth, neither stimulation nor inhibihon. The effect of E2 combined with MPA was more Obvious than either one used separately.E2 and MPA in combination with doxorubin showed a synergistic inhibition effect on OVCAR-3 cell growth, which suggesed that both of them could reverse the doxorubin resistance of OVCAR-3 cell line. The inhidition effect of Verapamil combined with doxorubin was obviously weaker than that of E2 and MPA. Conclusions E2 and MPA could reverse the doxorubin resistance of OVCAR-3 cell line and their inhibhon were more obvious than the of verapamil, which Suggested that E2 and MPA may be used clinically for adjuvant chemotherapy.
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
1999年第11期670-673,共4页
Chinese Journal of Obstetrics and Gynecology
基金
卫生部基金!96-1-280
关键词
雌激素
卵巢肿瘤
孕激素
OVCAR-3
耐药性
Estrogens Medroxyprogesterone Ovarian neoplasms Tumor cell, cultured Drug resistance neoplasm
