摘要
为阐明苯胺蓝染色检测苹果冷藏表皮病害的机理,以富士苹果(Malus domestica Borkh cv.Fuji)为试材,通过苯胺蓝染色和扫描电镜的方法,研究了苹果采收后苯胺蓝染色和0℃冷藏210 d后几种表皮病害的表皮结构。结果表明,(1)苹果表皮苯胺蓝染色部位存在皮孔发育不完善、表皮裂缝愈合不完善等质量缺陷,且该缺陷可以通过苯胺蓝染色的方法显现出来;(2)苹果贮藏210 d后表皮病害部位表皮及其蜡质层存在皮孔和表皮裂缝愈合不完善的质量缺陷;(3)苹果苯胺蓝处理后,发生染色部位往往与冷藏210 d后表皮病害发生部位相吻合。因此,苯胺蓝染色可用于检测果实贮藏病害的结构机理是:染色部位与病害部位存在共同结构基础,即发育不完善的表皮质量;该质量缺陷可用苯胺蓝染色的方法在果实入贮前使其显现出来。
In order to elucidate the mechanism on the detection of the apple superficial diseases or disorders after cold storage by aniline blue staining,the Fuji apple（Malus domestica Borkh cv.Fuji） was used as materials to study the structure of pre-storage apple stained with aniline blue and the structure of apple with the superficial diseases or disorders after 210 days of cold storage（0 ℃） by the method of scanning electron microscopy.The results revealed that the cuticular microcracking on stained portion of apple surface could be seen.The superficial disorders or diseases after cold storage might be related to the cuticular microcracking.In addition,the staining portion of apple surface after aniline blue staining was coincide with the portion of superficial disorders or diseases after cold storage.Then,it can be concluded that the cuticular microcracking was the same structure as diseases or disorders portion of apples and this cuticular microcracking could be seen by the method of pre-storage aniline blue staining.
出处
《果树学报》
CAS
CSCD
北大核心
2011年第4期703-707,F0004,共6页
Journal of Fruit Science
基金
山东省自然科学基金(Y2008D01)
关键词
苹果
苯胺蓝染色
表皮病害
扫描电镜
Apple
aniline blue staining
Superficial disorders or diseases
Scanning electron microscopy
