摘要
以蛋白去除率和多糖损失率为指标,比较Sevag法、三氯乙酸(trichloroacetic acid,TCA)法、木瓜蛋白酶法、木瓜蛋白酶-Sevag联用法和木瓜蛋白酶-TCA联用法脱除蚕蛹多糖蛋白的效果。结果显示:木瓜蛋白酶-TCA联用效果最好,其脱蛋白的最佳条件为木瓜蛋白酶用量[E]/[S]2.0%、酶解温度50℃、酶解反应1.5h、采用TCA法除蛋白1次,此时蛋白去除率为97.98%,多糖损失率为18.76%;紫外光谱扫描定性实验显示脱蛋白的多糖在260～280nm处只有少量吸收,表明大多数蛋白已去除。木瓜蛋白酶-TCA联用法是一种有效的多糖脱蛋白方法。
Protein removal rate and polysaccharide loss rate were used to evaluate and compare the effectiveness of Savag＇ s method, trichloroacetic acid （TCA） method, papain method and papain-TCA （papain hydrolysis followed by TCA precipitation） method in deproteinizing silkworm pupa polysaccharides. Papain-TCA method was found to be the best method for the deproteinization of silkworm pupa polysaccharides, and the optimal operating conditions were 1.5 h hydrolysis with papain at an enzyme-to-substrate of 2.0% and 50 ℃ followed by once treatment with TCA. A protein removal rate of 97.98% and a polysaccharide loss rate of 18.76% were achieved under such conditions. Only one absorption peak was observed in the UVvisible scanning spectrum of deporteinized silkworm pupa polysaccharides, indicating the removal of almost all proteins.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第14期21-24,共4页
Food Science
基金
广西自然科学基金项目(桂科自0728237)
广西壮族自治区教育厅科研项目(200607MS031)
广西大学基金项目(X071029)
关键词
蚕蛹
多糖
蛋白
脱除
pupa
polysaccharide
protein
removal
