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不同产地葛根的HPLC指纹图谱及质量评价研究 被引量:15

HPLC Fingerprints and Quality Evaluation of Radix puerariae from Different Places
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摘要 [目的]建立简单可行的葛根药材质量评价新方法。[方法]在进行方法学考察的基础上,采用高效液相色谱(HPLC)指纹图谱法分析不同产地葛根的指纹图谱,并采用相似度分析对不同产地的葛根药材进行了质量评价。[结果]测定了不同产地葛根药材中葛根素和大豆苷元2种有效成分的含量。不同产地的葛根药材质量差异较大,通过指纹图谱研究及相似度分析可以从整体上对葛根的质量进行评价。[结论]HPLC指纹图谱分析法可以为葛根药材的质量鉴别与质量控制提供可靠的依据。 [ Objective ] To develop a new and easy quality evaluation method for Radix puerariae. [ Method ] Based on the methodological study, the HPLC and similarity analysi method were adopted to respectively analyze the fingerprint and evaluate the quality of Radix puerariae from dif- ferent places. [ Result] The content of puerarin and daidzein in different Radix puerariae samples were determined, it was discovered that the quality of Radix puerariae varied largely from place to place, through fingerprint study and similarity analysis, the quality of Radix puerariae could be comprehensively analyzed. [ Conclusion] HPLC fingerorints provided reliable suDoort for the aualitv evaluation and control nf Radix puerariae.
出处 《安徽农业科学》 CAS 北大核心 2011年第18期10782-10784,10801,共4页 Journal of Anhui Agricultural Sciences
基金 国家自然科学基金资助项目(20975081)
关键词 葛根 葛根素 大豆苷元 HPLC指纹图谱 相似度分析 Radix puerariae Puerarin Daidzein HPLC fingerprints Similarity analysis
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参考文献3

  • 1ZHANG J L,CUI M,HE Y,et al. Chemical fingerprint and metabolic fin- gerprint analysis of Danshen injection by HPLC-UV and HPLC-MS meth- ods[J]. Journal of Pharmaceutical and Biomedical Analysis ,2005 ,36 :1029 -1035.
  • 2JI Y B,XU Q,HU Y Z,et al. Development,optimization and validation of a fingerprint of Ginkgo biloba extracts by high-performance liquid chromatog- raphy[J]. Jottmal of Chromatography A,2005,1066:97-104.
  • 3国家药典委员会.中华人民共和国药典:2005,-部[M].北京:化学工业出版社,2005:203,234.

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