摘要
目的:研究苓桂术甘汤对Aβ诱导小胶质细胞细胞生成和释放致炎因子IL-1β、IL-6及TNF-α的抑制作用。方法:体外培养BV-2小胶质细胞株,Aβ1-42诱导BV-2细胞活化,苓桂术甘汤不同浓度组(10×10-3、1×10-3、0.1×10-3、0.01×10-3、1×10-6、0.1×10-6 kg/L)进行干预,ELISA法检测各组IL-1β、IL-6及TNF-α含量。结果:与模型组比较,苓桂术甘汤组细胞上清中IL-1β、IL-6及TNF-α含量均有降低,以高浓度(10×10-3、1×10-3)最为显著(P<0.01)。结论:苓桂术甘汤对活化后BV-2细胞IL-1β、IL-6及TNF-α的的分泌有显著的抑制作用,良好的抗炎作用可能是苓桂术甘汤治疗老年性痴呆的重要依据和机理之一。
Objective：To study the inhibition effects of Ling Gui Zhu Gan Decoction on the production and release of proinflammatory cytokines IL-1β,IL-6 and TNF-α of microglia cells induced by Aβ.Methods：BV-2 microglia cell line was cultured,induced by Aβ1-42,and intervened with Ling Gui Zhu Gan Decoction of different concentrations（10×10-3,1×10-3,0.1×10-3,0.01×10-3,1×10-6,0.1×10-6）,then the contents of IL-1β,IL-6 and TNF-α were detected using the method of ELISA.Results：Compared with model group,the expression of IL-1β,IL-6 and TNF-α of Ling Gui Zhu Gan Decoction group decreased,and the high-concentration groups（10×10-3,1×10-3） decreased most significantly（P0.01）.Conclusion：Ling Gui Zhu Gan Decoction could inhibit the secretion of IL-1β,IL-6 and TNF-α of activated BV-2 cells significantly,the good anti-inflammatory effect may be the important basis and mechanism of the treatment of Alzheimer＇s Disease by Ling Gui Zhu Gan Decoction.
出处
《中医学报》
CAS
2011年第6期686-688,共3页
Acta Chinese Medicine
基金
江苏省普通高校研究生科研创新计划项目(编号:CX09B_267Z)
关键词
阿尔茨海默病
苓桂术甘汤
小胶质细胞
致炎因子
Alzheimer＇s Disease
Ling Gui Zhu Gan Decoction
microglia cells
proinflammatory cytokines
