摘要
目的：探讨新生期母婴分离对大鼠成年后行为的影响及其产生的机制。方法：建立动物模型,以受孕的w istar大鼠为研究对象,生产后,每笼饲养12只新生大鼠和1只哺乳期母鼠。实验分为2组,即母婴分离（NMS）组和对照组（N）,NMS组在出生后第2~21天每天同时间分离3 h,N组不做任何处理。在2月时,分别采用三通互通脑功能仪测定成年后大鼠行为的改变,并采用免疫组织化学的方法检测5-HT和5-HT受体在成年大鼠脑组织海马中的表达情况。结果：与对照组比较,母婴分离组成年后大鼠自发活动的总路程显著减少（P〈0.05）,平均速度减慢（P〈0.05）。与对照组比较,母婴分离组大鼠海马组织5-HT及5-HT受体的表达明显减少,差异具有统计学意义（P〈0.05）。结论：新生期母婴分离后可导致成年大鼠自发活动减少,表现出焦虑和抑郁样行为。5-HT及5-HT受体在海马中的表达减少是导致母婴分离后成年大鼠行为改变的重要机制。
Objective：To investigate the role and mechanism of neonate-mother separation on the behavior change in adult rats..Methods：The establishment of NMS animal models was done.The pregnancy Wistar rats was as the target,after production,then 10~12 per cage in neonatal rats and a lactating female rats.The experiments were randomly divided into 2 groups： neonatal maternal separation（NMS） group（3-hour daily maternal separation on postnatal day 2~21） and control group（no handling）.On the two months old,by ＂three links exchange apparatus of brain function＂ which was developed by Professor Feng zhiqiang of department of physiology in Luzhou Medical College,the changes of adult rat behavior were tested;the expression of 5-HT and 5-HT receptor in hippocampus were detected by immunohistochemical method..Results：Compared with control group,both the total distance and average speed of locomotor activity were significantly decreased（P0.05） in maternal separation group,and the expression of hippocampus 5-HT and 5-HT receptor were decreased obviously in maternal separation rats（P0.05）.Conclusion：Neonatal maternal separation could result in reduction in spontaneous activity of adult rats,showing anxiety and depression-like behavior.The expression of 5-HT and 5-HT receptor in the hippocampus after neonatal maternal separation could play an important role and mechanism in behavioral change.
出处
《中国妇幼保健》
CAS
北大核心
2011年第19期2984-2986,共3页
Maternal and Child Health Care of China
基金
泸州医学院青年基金和泸州市科技局重点项目〔2010-S-13(5/9)〕
