摘要
目的 研究急性脑梗死血小板蛋白激酶Ｃ（ＰＫＣ） 对血小板活性的影响及其致病作用。方法 利用单克隆抗体放射免疫法检测２０ 例急性脑梗死患者血小板膜及血浆内α颗粒膜蛋白（ＧＭＰ１４０） 的量；以血小板膜蛋白的磷酸化程度表达血小板ＰＫＣ 的活性。结果 患者组血小板膜及血浆内ＧＭＰ１４０ 量［ 分别为（１ ０７５ ±２３４） 分子数／ 血小板、（５－３ ±１－３１） 分子数×１０１０／ｍｌ］ 均较正常对照组［（７２４±２９８） 分子数／ 血小板、（１－４３ ±０－４８） 分子数×１０１０／ｍｌ］ 显著升高（ 均Ｐ＜ ０－００１） ；患者组血小板ＰＫＣ 基础活性（ 分子量为４７ ０００ 及２０ ０００ 蛋白质分别表达为４ １９０ 及２ ３１８ ｃｐｍ） 及活化活性（４７ ０００ 蛋白质于活化３ 分钟、１０ 分钟时表达为５ ３１９ 及７ ０１０ ｃｐｍ ；２０ ０００ 蛋白质对应时间表达为３ ２４７ 及４ ３０５ ｃｐｍ） 均较对照组（ 分子量为４７ ０００ 及２０ ０００ 蛋白质表达的基础活性分别为２ １１９ 及１ ９４２ ｃｐｍ ；４７ ０００ 蛋白质于活化３ 分钟、１０ 分钟时表达的活化活性为２ ６０８ 及３ ２１３ ｃｐｍ ，２０ ０００ 蛋白质对应时间表达的活化活性为２ ?
Objective To study platelet protein kinase C(PKC)′s effect on platelet activity and its role.Methods Platelet membrane and plasma α granule membrane protein (GMP 140) were measured in 20 patients with acute cerebral infarction by monoclonal antibody radioimmunoassay. The platelet PKC activity was expressed by the phosphorylation of platelet membrane protein.Results The GMP 140 level on platelet membrane [(1 075±234) molecules/platelet)] and in blood plasma [(5.3±1.31) molecules×10 10 /ml] for patient group (PG) were significantly elevated than those in control group (CG) [(724±298) molecules/platelet, (1.43± 0.48) molecules×10 10 /ml]( P <0.001). The basic activity and activated activity for various minutes for platelet PKC were significantly increased in PG than those in CG ( P <0.001), 4 190 cpm vs 2 119 cpm expressed by a protein of 47 000 molecular weight, 2 318 cpm vs 1 942 cpm expressed by a protein of 20 000 molecular weight, 5 319 cpm vs 2 608 cpm for 3 minutes and 7 010 cpm vs 3 213 cpm for 10 minutes expressed by 47 000 protein, 3 247 cpm Vs 2 489 cpm for 3 minutes and 4 305 cpm vs 2 824 cpm for 10 minutes expressed by 20 000 protein. There was a positive correlation between platelet PKC′ activity and GMP 140′ expression.Conclusion The high basic and activated activities for platelet PKC were the causes of unusual activated platelet and platelet thrombosis in acute cerebral infarction.
基金
辽宁省自然科学基金
关键词
脑梗塞死
血小板
蛋白激酶C
Cerebral infarction Blood platelet Protein kinase C Alpha granule membrane protein
