摘要
环介导等温扩增(LAMP)技术是近年来发展迅速的一种敏感、特异、方便快捷的核酸扩增技术。与传统PCR方法相比,LAMP技术属于等温扩增,无需昂贵的热循环仪,并且由于LAMP反应中产生大量的副产物-白色焦磷酸镁沉淀,扩增产物可不经过电泳,无需开盖直接通过肉眼观察即可判定结果,在反应前、后加入染色剂效果更佳。LAMP技术敏感性高、特异性强、操作简便,能够满足现场和条件较差的基层实验室进行快速检测的需求。近年来LAMP技术在寄生虫感染检测中应用广泛,本文对其研究进展进行了综述。
Loop-mediated isothermal amplification(LAMP) is a sensitive,specific,convenient and rapid DNA amplification technique that has developed rapidly over the past few years.Unlike conventional PCR,LAMP is an isothermal amplification assay that does not require an expensive thermocycler.Moreover,the LAMP reaction produces a large amount of magnesium pyrophosphate,a white precipitate by-product,that makes results easier to judge with the naked eye.If dye is added before or after reaction,the results are readily visible so further electrophoretic analysis is not needed.Therefore,LAMP is an efficient nucleic acid amplification assay with a high level of sensitivity and specificity;the technique is convenient and is suitable for rapid detection of parasitic diseases during field surveys or in poorly equipped laboratories.Numerous reports have described using LAMP to detect parasitic diseases.This paper reviews recent advances in the use of LAMP techniques to rapidly detect parasitic diseases.
作者
陈璐
吾拉木·马木提
CHEN Lu;MAMUTI-Wulamu(Department of Parasitology,School of Basic Medical Science,Xinjiang Medical University,Urumqi 830011,China;Hydatid Disease Key Laboratory,the first Affiliated Hospital of Xinjiang Medical University)
出处
《中国病原生物学杂志》
CSCD
2011年第6期469-471,共3页
Journal of Pathogen Biology
