摘要
报道了基于银纳米粒子构建的荧光传感平台,并用于核酸检测。此荧光传感平台对核酸检测基于以下策略:首先,荧光团标记的单链DNA探针被吸附到银纳米粒子的表面,荧光团与银纳米粒子近距离接触,发生荧光猝灭;加入与探针DNA序列互补的目标DNA,两者杂交形成双链DNA,并从银纳米粒子的表面脱离,荧光得到恢复。这种银纳米粒子构建的荧光传感平台对于完全互补和碱基错配的DNA序列具有良好的区分能力。
In this study,we reported Ag nanoparticles（AgNPs） as an effective fluorescent sensing platform for nucleic acid detection for the first time.The general concept used in this approach is based on adsorption of the fluorescently labeled single-stranded DNA（ssDNA） probe by AgNP,which is accompanied by substantial fluorescence quenching due to their close proximity,followed by specific hybridization with its target to form a double-stranded DNA（dsDNA）.The use of this sensing platform to differentiate complementary and mismatched sequences is also demonstrated.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2011年第7期998-1002,共5页
Chinese Journal of Analytical Chemistry
基金
中国国家重点基础研究发展计划(No.2011CB935800)资助
关键词
核酸检测
荧光传感平台
银纳米粒子
Nucleic acid detection
Fluorescent sensing platform
Silver nanoparticles
