摘要
目的：通过组织病理和测量血浆中ｖ Ｗ Ｆ的含量，了解皮肤撕脱伤后组织内微血管内皮细胞损伤的程度与血浆中ｖ Ｗ Ｆ（因子Ⅷ相关抗原）升高的关系，从而探讨皮肤撕脱伤后继发性坏死的原因． 方法：切片染色和双株ｖ Ｗ Ｆ单克隆抗体，以双抗夹心 Ｅ Ｌ Ｉ Ｓ Ａ（酶联免疫吸附法）技术测定血浆中ｖ Ｗ Ｆ含量． 结果：与对照组相比，皮肤撕脱伤后血浆中ｖ Ｗ Ｆ开始升高，３ ｄ 时达到峰值，７ ｄ 时仍高于对照组，与病理检查相吻合，ｖ Ｗ Ｆ含量与内皮细胞损伤成正相关． 结论：血中ｖ Ｗ Ｆ的含量变化能反映皮肤撕脱伤继发性损伤的程度．
AIM: To identify the relations between the extent of EC injury in skin tissue after avulsion and raising vWF in plasma, and study the cause of the secondary necrosis after avulsion through measuring the changes of vWF and pathology. METHODS: HE dye of tissue slice and ELISA technique were used. RESULTS: vWF level in plasma gradually increased after avulsion, peak value was in the third day, which coincided with pathology and the extent of EC injury. CONCLUSION: The changes of vWF in plasma can explain the extent of the secondary injury after avulsion.
出处
《第四军医大学学报》
1999年第9期804-805,共2页
Journal of the Fourth Military Medical University
关键词
皮肤撕脱伤
内皮细胞
Factor Ⅷ related antigen(vWF)
skin avulsion injury
endothliocyte
