摘要
用等位基因特异MVR-PCR技术能够对小卫星区域MS31(D7S21位点)等位基因结构的多态性进行分析,在法医学个体识别上具有极其重要的作用。对100名中国人无关个体MS31A5'端侧翼DNA多态性(-4A/G、-109C/T和-221G/C)进行分析,获得了其不同等位基因的频率和单倍体型的分布,结果表明81.8%的中国人DNA样品能够用等位基因特异MVR-PCR分析。
Allele-specific MVR-PCR can be Performed on genondc DNA to sample the diversity of allelic structure at MS31A (D7S21 locus). Three polymorphic posihons in MS31A 5' flanking DNA were typed from 100 unrelatod Chinese in this study. The results showed that single alleies could be mapped from genomic DNA in above 80% samples of Chinese in this locus. The distribution of haplotypes of unrelated Chinese was also reported in this paper.