摘要
建立了以羧甲基-β-环糊精为手性流动相添加剂,在反相液谱条件下拆分巴氯芬对映体的方法。研究了手性添加剂的种类及浓度、流动相组成、pH值及流速等因素对拆分的影响。流动相为甲醇、乙腈、水,体积比为90:5:5,其中含2.00 g/L CM-β-CD,pH值5.95,流速0.60 mL/min,检测波长231 nm,分离度为1.31。该方法简便、快速、相对偏差小。
The method for the separation of baclofen enantiomers was developed using reversed phase high-performance liquid chromatography with carboxymethyl-β-eyclodextrin as chiral mobile phase additive. The effects of concentration of chiral additive, organic solvents （ type and concentration ） , pH value and flow rate on the enantiomers separation was studied systematically The best separation was obtained with methanol-acetonitrile-water （ 90 ： 5 ： 5, pH 5.95 ） mixed with 2.00 g/L CM-β-CD as mobile. The flow rate was 0.60 mL/min. The detection wavelength was 231 nm. The resolution of enantiomers was 1.31. The method was simple and fast with small relative standard deviations.
出处
《齐齐哈尔大学学报（自然科学版）》
2011年第4期60-62,共3页
Journal of Qiqihar University(Natural Science Edition)
