摘要
目的探讨ATP生物荧光体外药敏检测法(ATP-TCA)的特点及其在中晚期结直肠癌患者化疗方案中的指导价值。方法应用ATP-TCA体外检测59例结直肠癌细胞对常用抗癌药物的敏感性。结果 ATP-TCA法对结直肠癌标本的可评估率为96.61%。57例结直肠癌细胞对4组联合化疗药物氟尿嘧啶+丝裂霉素、氟尿嘧啶+奥沙利铂、氟尿嘧啶+伊立替康、氟尿嘧啶+奥沙利铂+伊立替康相比氟尿嘧啶、丝裂霉素、伊立替康、奥沙利铂4种单药的敏感度有高度显著性差异(P=0.0006)。应用ATP-TCA检测结果指导中晚期结直肠癌患者化疗,临床近期有效率为59.65%(34/57),总预测准确率为63.16%(36/57),阳性符合率为61.82%(34/55),阴性符合率为100%(2/2)。结论 ATP-TCA能有效检测化疗药物的敏感性,对指导中晚期结直肠癌患者化疗有重要的临床意义。
Objective To explore the experimental characteristics of adenosine triphosphate based tumour chemosensitivity assay（ATP-TCA） and its guiding value in the screening of chemotherapeutic agents for advanced colorectal cancer.Methods The sensitivity of 8 groups of common chemotherapeutic drugs in 59 patients with advanced colorectal cancer was tested by ATP-TCA in vitro.Results The evaluable rate in the specimens of colorectal cancer was 96.61% by ATP-TCA.There was significant difference（P=0.0006）in the sensitivity between the 4 groups of combination regimens（fluorouracil＋mitomycin,fluorouracil＋oxaliplatin,fluorouracil＋irinotecan and fluorouracil＋oxaliplatin＋irinotecan） and 4 single drugs（fluorouracil,mitomycin,oxaliplatin and irinotecan） in 57 cases of colorectal cancer.The recent effective rate was 59.65%（34/57）according to the results of ATP-TCA in directing chemotherapy for advanced colorectal cancer,the total predicting accuracy rate was 63.16%（36/57）,the positive coincidence rate was 61.82%（34/55） and the negative coincidence rate was 100%（2/2）.Conclusion ATP-TCA technique is an effective method in detecting chemosensitivity,and has important clinical value to guide postoperative chemotherapy for advanced colorectal cancer.
出处
《临床肿瘤学杂志》
CAS
2011年第6期538-541,共4页
Chinese Clinical Oncology
