摘要
目的探讨盲肠结扎穿孔脓毒症大鼠肺泡巨噬细胞(AM)和血管内皮细胞变化情况及其相关性。方法 60只SD大鼠随机分成A、B两组,A组仅行盲肠探查术,B组采用盲肠结扎穿孔术(CLP)制作大鼠脓毒症模型。各组均于术后3、6、12、24、48h时相点,分别留取肺泡灌洗液检测巨噬细胞炎症蛋白-1α(MIP-1α)水平,留取血清检测肿瘤坏死因子-α(TNF-α)和E-选择素,并统计学分析肺泡灌洗液MIP-1α与血清TNF-α和E-选择素变化的相关性。结果①CLP术后3 h B组肺泡灌洗液MIP-1α和血清TNF-α、E-选择素即开始升高,明显高于A组,且逐步升高,到术后12 h达峰值,随后逐渐下降,但在48 h时点仍然显著高于A组,差异有统计学意义(P<0.05,P<0.01)。②B组肺泡灌洗液MIP-1α与血清TNF-α和E-选择素变化分别呈显著正相关。结论 CLP脓毒症致急性肺损伤(ALI)后,AM的变化可以通过E-选择素影响肺血管内皮细胞变化,AM聚集与血管内皮细胞损伤在脓毒症致ALI过程中发挥重要作用。
Objective To study of changes and correlation of alveolar macrophage（AM） inflammatory protein-1α（MIP-1α） level and vascular endothelial cells in septic rats of cecal ligation and puncture（CLP）.Methods 60 SD rats were divided into two groups randomly,group A sham operation group,and group B was sepsis group.Rats of group A only were appendix exploration.the rats received CLP in group B.In each group,the subcutaneous injected saline of 5 ml at the time of respectively.In group A and B,all rats were detected the levels of MIP-1α through bronchial alvolar lavage fluid,E-selectin and TNF-α in serum at 3,6,12,24,48 h.Results ① Compared with group A,the levels of MIP-1α in bronchial alvolar lavage fluid,E-selectin,TNF-α in blood serum were increased significantly in group B（P0.05）.②The Bronchial Alvolar Lavage Fluid MIP-1α and serum E-selectin,TNF-α had obviously correlation.Conclusion In the process of sepsis-induced ALI,the collection of AM and the damages of vascular endothelial have obviously direct correlation.It hints that AM and vascular endothelial may play an important role in sepsis-induced ALI.
出处
《安徽医科大学学报》
CAS
北大核心
2011年第7期645-647,共3页
Acta Universitatis Medicinalis Anhui
基金
安徽省卫生厅科研基金(编号:09A067)
