摘要
目的对日本血吸虫尼克酰胺磷酸核糖转移酶(SjNAMPT)基因序列及蛋白序列进行生物信息学分析,为该基因的克隆表达和开发应用奠定基础。方法利用Internet在线分析程序和相关工具软件分析SjNAMPT基因的开放阅读框,分析基因编码蛋白的理化性质、结构域,并预测其空间结构和功能。结果 SjNAMPT蛋白由179个氨基酸组成,分子量为19.63kDa,理论等电点为7.64,为稳定性蛋白,无跨膜区和螺旋卷曲结构,不含有信号肽,含有多个磷酸化位点,该蛋白属于磷酸核糖转移酶保守结构域家族,亚细胞定位于细胞质,二级结构以无规卷曲为主,SjNAMPT蛋白序列与其他物种同种蛋白序列相似性较低。结论 SjNAMPT蛋白为日本血吸虫特异性蛋白,具有一定酶活性,可能与日本血吸虫嘌呤代谢和糖代谢有关,值得进一步研究。
Objective To perform the bioinformatics analysis of nicotinamide phosphoribosyl transferase of Schistosoma japonicum so as to provide the evidence for the development and application of it. Methods Internet online procedures and the related software were exploited to analyze the open reading frame （ORF） of SjNAMPT gene, physical and chemical properties of SjNAMPT protein and the domains of the protein, and predict the space structure and functions of the protein. Results The SjNAMPT protein sequence was composed of 179 amino acids with 19.63 kDa of the molecular weight. The protein was stable and had no structures of transmembrane and helical coils. The protein belonged to the family of nicotinate phosphoribosyl transferase with multiple phosphorylation sites. The protein probably located in cytoplasm and dominated by random coils in second structure. The similarity of the SjNAMPT protein sequence was lower than that of other species of NAMPT. Conclusions SjNAMPT is a specific protein of Schistosoma japonicum with a certain activity, and probably related to purine metabolism and glucose metabolism. It is worth for further study.
出处
《中国血吸虫病防治杂志》
CAS
CSCD
北大核心
2011年第3期292-295,共4页
Chinese Journal of Schistosomiasis Control
基金
国家高技术研究发展计划(863计划)目标导向类课题(2007AA02Z153)
关键词
日本血吸虫
尼克酰胺磷酸核糖转移酶
生物信息学
疫苗
Schistosoma japonicum
Nicotinamide phosphoribosyl transferase
Bioinformatics
Vaccine
