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大鼠皮质骨上机械性显微损伤的修复机制研究 被引量：1

Repairing mechanism of mechanical microdamage in the cortical bone of rats
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摘要 目的研究大鼠皮质骨上植入物造成的机械性显微损伤的修复机制。方法 30只SD大鼠随机分为两组,一组行卵巢切除术,另一组行假手术。3个月后在右侧胫骨行钻孔术,分别于钻孔后1、2、4周后处死,处死前注射四环素和钙黄绿素进行标记。将含有钻孔的骨段(1 cm)应用大块碱性品红染色,甲基丙烯酸甲酯包埋后切成约50μm厚的切片。应用Bioquant图象分析系统对切片进行骨形态计量学分析。结果去卵巢大鼠和假手术大鼠都出现了与显微损伤有关的骨吸收腔,其中去卵巢组的孔隙率和骨吸收腔明显高于假手术组(P<0.05);随着术后时间的延长,吸收腔的数目逐渐增加,各时间点之间的差异有明显的统计学意义(P<0.05)。结论去卵巢大鼠的孔隙率和骨吸收腔数明显高于假手术大鼠,反映了在雌激素缺乏和较多裂纹形成两种因素刺激下,去卵巢大鼠皮质骨内的重建更为活跃,这会降低骨的强度从而增加骨折的危险性。 Objective To study the repairing mechanism of mechanical microdamage around implants in the cortical bone of rats.Methods Thirty rats were divided into the ovariectomy group（OVX） and the sham group.At three months after the ovariectomy,a hole was drilled in the right tibial diaphysis by a metal pin.The rats were executed at 1,2 and 4 weeks,respectively,after the hole drilling.The tetracycline and calcein labeling were performed before the execution.Bone segments containing the hole were stained with the basic fuchsin,embedded in the methylmethacrylate and cut into sections with thickness of 50 μm.Histomorphometric measurement was conducted on bone sections using Bioquant image analysis system.Results Bone resporpion cavities related to the microdamage occurred in both the OVX rats and the sham-operated rats.The bone porosity and the number of bone resorption cavities were both greater in the OVX rats than that in the sham-operated rats（P0.05）.In addition,the number of bone resorption cavities significantly increased with time after the surgery（P0.05）.Conclusions Increased bone porosity and resorption cavities in OVX rats may be related to the crack formation and the estrogen deficiency,which made the bone remodeling in the cortical bone of OVX rats more active.However,remarkably increased resorption cavities would reduce the bone strength and increase the risk of bone fracture.
作者 宋国路 于志锋 裘世静 汤亭亭
机构地区 上海交通大学医学院附属第九人民医院骨科上海市骨科内植物重点实验室 Boneand Mineral Research Laboratory
出处 《医用生物力学》 EI CAS CSCD 2011年第3期252-255,285,共5页 Journal of Medical Biomechanics
基金 上海市科委项目(09dz2200400,08411950500) 国家青年自然科学基金项目(11002090) 上海市自然科学基金项目(10ZR1417900) 上海市教委重点学科建设基金(J50206)
关键词 机械性显微损伤 骨重建 皮质骨 孔隙率 裂纹 骨折 大鼠 Mechanical microdamage Bone remodeling Cortical bone Porosity Cracks Fracture Rats
分类号 R318.01 [医药卫生—生物医学工程]
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参考文献13

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二级参考文献28

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共引文献3

同被引文献29

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医用生物力学
2011年 第3期

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