摘要
目的：研究实验性肝癌的组织发生过程中甲胎蛋白的表达，旨在为肝癌诊断奠定形态学基础。方法：Ｗｉｓｔａｒ大白鼠喂００４％３′ＭｅＤＡＢ不同日程处死，３６周处死完，观察动力形态变化和免疫组化ＡＦＰ表达。结果：实验早期卵圆细胞增生，该细胞是多向分化的干细胞。它进一步分化成过渡细胞、胚胎性小肝细胞，后者可呈不典型增生，它们散在或群集，这些细胞ＡＦＰ强阳性，群集不典型增生的小肝细胞形态上不同于肝细胞增生结节，前者和肝细胞癌形态相似。卵圆细胞向胆管分化形成纤维腺瘤样增生，其上皮细胞也见不典型增生。５８只实验动物生成２６例肝癌，其中肝细胞癌１８例，混合癌８例，癌ＡＦＰ强阳性，其周围宿主肝细胞和实验晚期无癌动物的肝细胞也见程度不同的ＡＦＰ阳性表达。
Purpose To investigate the carcinohistogenesis of hepatocarcinoma and AFP expression in oncogenesis and to lay a morpholoogical foundation for diagnosing and curing hepatocarcinoma. Methods Wistar rats were fed on 004% 3MeDAB and killed according to different dates. All the animals were killed during 36 weeks. The morphologic changes of dynamics and expression of alpha fetoprotein (AFP) were observed with immunohistochemical method. Results Hyperplasia of oval cells occurred in the early experimemtal stage and the oval cells were multipotential stem cell. They further differentiated into transitional cell and embryoid small liver cell, and the latter may form atypical hyperplasia, scattered or crowded. AFP in these cells appeared to be strongly positive expression. The feature was different between dysplastic small hepatocytes with crowd and proliferative nodules of hepatocytes. The former was similar to hepatocytic carcinoma morphologic ally. The oval cells differeatiated towards biliary duct to fotoid structure, and atypical hyperplasia was seen in their epithelial cells. 26 of 58 experimental animals were cases of hepatocarcinoma, among whom 18 cases of hepatocytic carcinoma and 8 mixed carcinoma were found. The hepatocellular carcinoma showed stuongly AFP positive expression. In the host hepatocytes around cancer and hepatocytes of nonneoplastic animals of later experimental period also appered to be various degrees of positive expression of the AFP. Conclusion The precancerosis of hepatocellular carcinoma and bile duct caricnoma is atypical hyperplasia of small hepatocytes and epithelial cells of bile canaliculi.
出处
《临床与实验病理学杂志》
CAS
CSCD
1999年第3期224-226,I035,共4页
Chinese Journal of Clinical and Experimental Pathology
关键词
肝肿瘤
甲胎蛋白
大鼠
癌组织
liver neoplasms, experimental
hyperplasia
alphafetoproteins
ratsorm fibroadenoma
