摘要
参考Friedman等分离人肝脏星形细胞(HSC)的方法,采用校正密度的淋巴细胞分离液进行一步梯度离心,成功地分离得到了正常人HSC。HSC的收获量约为1×10~7个/10克肝脏组织,存活率在95％以上,纯度达85％以上。传代后纯度接近100％。对人HSC的标志物进行检测发现,结蛋白不宜作为鉴定初分离的及原代培养初期人HSC纯度的指标,而α-平滑肌肌动蛋白可作为鉴定激活的人HSC的可靠指标。
According to the method described by Friedman et al. ,a modified procedure for isolation and culture of human hepatic stellate cells(HSC) was established with centrifugation on a single-layer density cushion of Ficoll-Hypaque(ρ = 1. 050). The yield of HSC using this method was about 1×107 per 10 grams of li ver. Viability of the cells was more than 95% and purity was more than 85%. After the first passage.the purity of HSC was nearly 100%. It was found that desmin staining could not be used as a marker for identification of human HSC and α-smooth muscle actin staining could be used as an ideal marker for identification of activated human HSC.
出处
《细胞生物学杂志》
CSCD
1999年第3期I000-I000,共1页
Chinese Journal of Cell Biology
关键词
肝脏
星状细胞
分离
培养
鉴定
Hepatic stellate cells Cell isolation Cell culture Identification
