摘要
目的探讨并评价全自动血型分析仪应用于献血者血型筛查和盐水不规则抗体检测。方法采用全自动血型分析仪(全自动法)对25 554例献血者标本作ABO及RhD血型鉴定、盐水不规则抗体初筛,并与加样仪加样手工比色法(半自动法)作比对实验。ABO正反定型不一致而无法定型、O细胞凝集、RhD阴性的标本送血型红细胞参比实验室鉴定。结果全自动法与半自动法比较,ABO、RhD阴性血型1次准确定型率:99.93%(25 535/25 554)vs99.95%(25 542/25 554)(P>0.05);O细胞凝集阳性率:0.18%(46/25 554)vs0.10%(26/25 554)(P<0.05),经参比实验室确认,盐水不规则抗体分别为43、24例(0.17%vs0.09%,P<0.05);正反定型不符:17例(0.06%)vs10例(0.04%),参比实验室确认,经参比实验室确认2种方法共同拥有亚型5例,亚型漏检各2例(0.01%),余为正常血型(10/17vs3/10,P>0.05)。结论全自动血型分析仪作ABO、RhD血型筛查的技术相关性好、重复性好;除了全自动化、操作规范化、标准化等优点外,全自动血型分析仪更易发现盐水不规则抗体。
Objective To evaluate the performance of a fully automated blood grouping analyzer for ABO and RhD screening and the red blood cell IgM unexpected antibody detection.Methods A total of 25 554 samples were collected from blood donors.ABO,RhD,and IgM unexpected antibodies were tested simultaneously by fully automated blood grouping analyzer and manual colorimetric method with semi-automated sampler.For discrepancies between forward and reverse ABO grouping,agglutination of O cells,RhD negative results,samples would be referred to the reference laboratory of Shanghai Blood Center for further identification.Results The accuracy rates of ABO grouping by fully automated blood grouping analyzer and manual colorimetric method with semi-automated sampler were 99.93%（25 535/25 554） and 99.95%（25 542/25 554）respectively（P0.05）;the rates of agglutination of O cells were 0.18%（46/25 554）,and 0.10%（26/25 554）（P0.05） respectively;the ABO forward and reverse grouping discrepancies were 17（0.06%） and 10（0.04%）,respectively.Reference lab confirmed that there were 5 subgroups discovered by both methods;2 subgroups were missed by each method（0.01%）,the rest were normal ABO blood group specimens（10/17 vs 3/10,P0.05）.Conclusion The fully automated blood grouping analyzer can perform blood donor testing with high accuracy,high standardization in operation,and easier identification of IgM irregular antibodies.
出处
《中国输血杂志》
CAS
CSCD
北大核心
2011年第5期395-398,共4页
Chinese Journal of Blood Transfusion
