摘要
利用简单重复序列（simple sequence repeat，SSR）分子标记技术对云南省分布的8个大花香水月季天然居群遗传性进行了分析，结果表明：所选用的12对SSR引物，共扩增出72个位点，其中多态性位点70个，占总条带数的97．22％；在物种水平上，总居群的Nei’s基因多样性指数（He）和香农指数（I）分别为0．3012和0．4600，说明云南地区大花香水月季居群的遗传多样性较高；大花香水月季居群内遗传变异（69．49％）大于居群间遗传变异（30．51％），说明居群内变异是其居群的主要变异来源；利用Popgene计算出两两居群间的Nei＇s遗传一致度（I）和遗传距离（D），其范围分别为0．7615～O．9348和0．0674～O．2724，再依据遗传距离进行聚类分析，结果表明居群遗传距离与地理位置无显著的相关性。
The genetic diversity of eight natural populations of Rosa odorata Sweet var. gigantea Rehd. et Wils. in Yunnan Province was analyzed with SSR markers. The result showed that 72 bands were amplified with twelve polymorphic primers. Polymorphic loci were 70 and percentage of polymorphism was 97. 22%. At the level of species,the Nei＇s genetic diversity was 0. 301 2 and the Shannon information index was 0. 460 0, showing that the genetic diversity of the populations of Rosa odorata Sweet var. gigantea in Yunnan Province was abundant. It was 69.49% of genetic variation within populations, and 30. 51% of genetic variation among populations. So the genetic variation within populations was the main reason of the genetic variation. The Nei＇s pairwise genetic identity and genetic distance were 0. 761 5-0. 934 8 and 0. 067 4-0. 272 4. The cluster analysis based on genetic distance showed that the genetic distance was not significantly related with the geographic location.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2011年第3期300-304,共5页
Journal of Huazhong Agricultural University
基金
国家自然科学基金项目(30660117)
国家"863"计划子课题项目(2006AA100109)
国家科技支撑计划子课题项目(2006BAD01A1805)
公益性行业(农业)科研专项(200903020)
云南省科技创新强省计划项目(2007C0003Z)
关键词
大花香水月季
遗传多样性
简单重复序列(SSR)
Rosa odorata Sweet var. gigantea Rehd. et Wils.
genetic diversity
simple sequence repeat
