摘要
目的建立C57BL/6颞叶癫痫小鼠模型,观察其在行为学和病理学上的变化。方法将C57BL/6小鼠随机分为空白对照组、0.9%氯化钠注射组(35μL/g)和海人酸注射组(12 mg/kg),进行单侧海马注射。注射5 d或5周后,取小鼠脑进行HE染色,观察海马病理结构改变;检测海马组织中mTOR通路标志物P-S6蛋白质表达量的改变。结果 (1)海人酸注射小鼠发生面部抽搐、双侧前肢痉挛等急性期癫痫持续发作和慢性期自发发作;(2)海马注射侧CA1、CA3神经元明显丢失,慢性期小鼠模型出现中齿状回颗粒细胞散布,符合海马硬化特征性病理改变;(3)小鼠海马组织中P-S6蛋白质的表达量在急性期(P<0.05)和慢性期(P<0.01)均上调。结论单侧海马注射海人酸可成功建立C57BL/6小鼠内侧颞叶癫痫模型,可观察到与内侧颞叶癫痫临床表现类似的行为学、组织病理学上的改变。
Objective To establish the kainic acid（KA）-induced mesial temporal lobe epilepsy（TLE） mice model and detect the characteristic changes.Methods Healthy male C57BL/6 mice were randomly divided into control group（non-injected）,saline group（35 μL/g） and KA group（12 mg/kg） for intrahippocampal injections.5 days and 5 weeks after injection,the mice brains were sectioned and stained by hemotoxylin-eosin（HE） to detect the pathologic changes in the hippocampal area;the expression level of P-S6 protein,the marker of hippocampus mTOR signal pathway,was also determinated to verify the animal model.Results（1）Only KA-treated mice showed wet dog shakes,facial clonus and generalized tonic-clonic convulsions.（2）After injection of KA,neuronal cell loss was prominent in in CA1,CA3 and hilar area of the ipsilateral hippocampus;granule cell in dentate gyrus area dispersed,which are fertures of hippocampus sclerosis.（3）P-S6 expression level increased in both acute（P0.05） and chronic phases（P0.01） of MTLE models.Conclusion Intrahippocampal injection of KA inC57BL/6 mice could induce behavioural,histopathological changes which are similar to those clinic features of MTLE.
出处
《基础医学与临床》
CSCD
北大核心
2011年第7期820-826,共7页
Basic and Clinical Medicine
基金
国家自然科学基金(30971001
30721063)
北京自然科学基金(7102109)
霍英东教育基金(121024)
