摘要
目的 研究荧光原位杂交技术（ＦＩＳＨ） 在检测异常染色体中的应用价值及其意义。方法 用ＦＩＳＨ方法和染色体Ｇ分带技术对比观察急性淋巴细胞白血病（ＡＬＬ） 患者染色体的变化。结果 对３８ 例患者１７ ，１８ 和２１ 号染色体进行Ｇ分带技术检查，结果分别有１１，１３ 和１８ 例患者细胞染色体存在三倍体细胞。ＦＩＳＨ 检查结果，除上述病例发现三倍体细胞外，染色体Ｇ分带检查正常者中也有部分病例存在三倍体。结论 ＦＩＳＨ检测异常染色体，方法简便、灵敏、可靠，与常规细胞遗传学方法比较，ＦＩＳＨ 方法不需要培养细胞，对分裂期和分裂间期细胞均可进行基因定位检测，尤其在有核细胞少等不适于进行细胞遗传学分析的情况下，ＦＩＳＨ 方法仍可得到有意义的数据。
Objective To investigate the value of fluorescence in situ hybridization (FISH) in detecting aberrant chromosomes in acute lymphoblastic leukemias. Methods Conventional banding techniques and FISH were used to detect trisomy 17,18 and 21 in 38 patients with acute lymphoblastic leukemia.Lymphoid DNA probes specific for chromosomes no.17,18 and 21 were used for FISH. Results Out of the 38 patients,18 had +17,22 +18 and 23 +21.The number of trisomy cells ranged from 7% to 80%. Ten normal bone marrow samples were hybridized with the chromosomes 17,18 and 21 probes.Conclusion FISH is more sensitive than conventional cytogenetic analysis and allows systematic study on large cohort of patients without the need of metaphase preparations.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
1999年第12期640-642,共3页
Chinese Journal of Hematology
关键词
荧光原位杂交
白血病
DNA探针
ALL
In situ hybridization,fluorescence Chromosomal abnormalities Leukemia,lymphoblastic,acute DNA probes
