摘要
目的筛选人牙髓细胞(human dental pulp cells,HDPCs)在未分化状态及在矿化诱导液中差异表达的miRNA并预测其靶基因。方法体外分离培养临床就诊患者来源的人牙髓细胞并鉴定后,分别在普通培养基和矿化诱导培养基中培养,用矿化诱导液诱导细胞向成牙本质细胞方向分化,对细胞结节形成情况进行观察并用Vonkossa染色确定HDPCs分化情况。取培养21 d的细胞用miRNA芯片检测差异表达的miRNA,并通过miRGen数据库预测其靶基因,通过GO和KEGG Pathway进行靶基因功能富积分析。结果矿化诱导的HDPCs较未诱导的HDPCs,有72种miRNA发生1.5倍以上表达上调,61种miRNA发生1.5倍以上表达下调。通过miRNA靶标预测工具分析发现,带有靶标基因的miRNA有35个,35个miRNA的靶标基因总和1 327个,miRNA和靶标基因关系对总共2158个。结论成功筛选出HDPCs miRNAs表达谱,预测到部分靶基因。
Objective To screen the differential expression microRNAs in human dental pulp cells under the undifferentiation condition and in the mineralization induction fluid,and forecast target genes.Methods After human dental pulp cells were isolated from outpatients and then identified,these cells were separately cultured in the ordinary culture medium or the mineralization induction culture medium.The formation of cellular nodules were observed,and the cell differentiation was observed by Vonkossa staining.The differential expression of microRNA was detected by microRNA chip,and target genes were forecast by miRGen database.Functional rich products of these target genes were assayed by GO and KEGG Pathway.Results Compared with the human dental pulp cells cultured in the ordinary culture medium,there were 72 microRNAs expressing over 1.5-fold up-regulated and 61 microRNAs expressing over 1.5-fold down-regulated in the mineralization induction culture medium.There were 35 miRNAs with target genes by microRNA target forecast tool analysis.The total target genes of 35 miRNAs was 1 327,and the total relations of miRNA and target gene were 2 158.Conclusion We screen miRNAs signature of human dental pulp cells and forecast some target genes.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2011年第11期1132-1135,共4页
Journal of Third Military Medical University
基金
重庆市卫生局科研基金(2008-2-253)~~
