摘要
目的探讨埃扎伊尔-埃博拉病毒(Zaire Ebolavirus)包膜蛋白(GP)DNA疫苗诱导小鼠产生中和抗体的特性及研制埃博拉病毒DNA疫苗的可行性。方法构建埃博拉病毒包膜蛋白GP真核表达质粒Peak13CD5LGP,采用100μg剂量的质粒免疫小鼠,以纯化的埃博拉病毒包膜蛋白亚单位融合蛋白(GP1-Fc)作为抗原,通过ELISA检测及Western blot验证测定血清抗体滴度及特异性。结果 100μg质粒免疫小鼠3次后,小鼠血清中GP中和抗体滴度达到1∶2 000。结论编码埃博拉病毒包膜蛋白GP的DNA疫苗(pEAK13CD5LGP)能够诱导小鼠产生高滴度和持久的中和抗体,作为埃博拉病毒疫苗具有潜在的应用价值。
Objective To characterize of neutralization antibody in mice induced by DNA vaccine encoding Zaire Ebolavirus glycoprotein（GP） and to develop DNA vaccine against Ebola virus.Methods Zaire Ebolavirus GP encoding plasmid Peak13CD5LGP was constructed.100 μg dosage of Peak13CD5LGP was used to immunize the mice,the titer of neutralization antibody against Zaire Ebolavirus glycoprotein was assayed by ELISA using Zaire Ebolavirus glycoprotein subunit GP1-Fc fusion protien as capture antigen.Results After boost immunization with 100 μg dosage Peak13CD5LGP plasmid,the titer of neutralization antibody of anti-GP was 1∶ 2 000,both efficiency and specificity of neutralization antibody was verified by western blot.Conclusion 100 μg dosage of DNA vaccine encoding Zaire Ebolavirus envelope glycoprotein is able to induce persistent and high level of neutralizing anibody,and may be a potential vaccine against the deadly Zaire Ebolavirus.
出处
《基础医学与临床》
CSCD
北大核心
2011年第6期667-671,共5页
Basic and Clinical Medicine
基金
国家自然科学基金(30625013)
关键词
埃博拉病毒
包膜蛋白
DNA疫苗
Zaire Ebolavirus
envelope glycoprotein
DNA vaccine
