摘要
核磁共振(nuclear magnetic resonance,NMR)是以原子核自旋的共振跃迁为探测对象的谱学方法.当自旋量子数不为零的原子核处于外磁场中时,会引起能级的Zeeman分裂.若再施加能量等于Zeeman能级差的射频场,则会诱发原子核自旋的共振跃迁,这种现象即为核磁共振.核自旋的共振频率与原子的类型有关,且受原子所处化学和物理环境的影响.此外,NMR能量较低,不会影响探测对象(常为分子)的状态.因此,NMR能够在无损条件下提供多种具有原子和分子分辨的物质组成、结构、形态、动态变化等丰富信息.
Nuclear magnetic resonance（NMR）spectroscopy is a state-of-the-art technology which has been widely applied in biological systems over the past decades.It is a powerful tool for macromolecular structure determination in solution,and has the unique advantage of being capable of elucidating the structure and dynamic behavior of proteins during vital biomedical processes.In this review,we introduce the recent progress in NMR techniques for studying the structure,interaction and dynamics of proteins.The methods for NMR based drug discovery and metabonomics are also briefly introduced.
出处
《物理》
CAS
北大核心
2011年第6期366-373,共8页
Physics
基金
国家自然科学基金(批准号:20921004
90813017)
国家重点基础研究发展计划(批准号:2009CB918600)资助项目
关键词
核磁共振
蛋白质溶液结构
蛋白质动力学
药物识别
nuclear magnetic resonance（NMR）
protein solution structure
protein dynamics
drug screen
