摘要
目的 探讨单胺氧化酶 Ｂ（ Ｍ Ａ Ｏ Ｂ） 抑制剂对衰老模型小鼠的抗氧化作用。方法 分别采用化学发光法、二硫对硝基苯甲酸（ Ｄ Ｔ Ｎ Ｂ） 法、过氧化氢酶试剂盒法和荧光法检测丙炔苯丙胺（ｓｅｌｅｇｉｌｉｎｅ） 和２己基 Ｎ甲基丙炔胺（２ Ｈｅｘｙｌ Ｎｍｅｔｈｙｌｐｒｏｐａｒｇｙｌａｍｉｎｅ ，２ Ｈｘ Ｍ Ｐ） 对 Ｄ半乳糖诱导的衰老模型小鼠（１１ 只） 脑和肝铜／ 锌超氧化物歧化酶（ Ｃｕ／ Ｚｎ Ｓ Ｏ Ｄ） 、谷胱甘肽过氧化物酶（ Ｇ Ｓ Ｈ Ｐｘ） 、过氧化氢酶活力和脂褐素含量的影响。结果 与衰老模型组相比较，ｓｅｌｅｇｉｌｉｎｅ（０２５ ｍｇ／ｋｇ） 组（１２ 只） 和２ Ｈｘ Ｍ Ｐ（１０ ｍｇ／ｋｇ） 组小鼠（１２ 只） 脑、肝 Ｃｕ／ Ｚｎ Ｓ Ｏ Ｄ 和肝过氧化氢酶活力均高于衰老模型组（ Ｐ＜ ００１） ，肝脂褐素水平低于衰老模型组（ Ｐ＜ ００５） ，２ Ｈｘ Ｍ Ｐ 组脑（ Ｐ＜ ００１） 和肝（ Ｐ＜ ００５） Ｇ Ｓ Ｈ Ｐｘ 活力也高于衰老模型组；２ Ｈｘ Ｍ Ｐ 组脑、肝 Ｇ Ｓ Ｈ Ｐｘ 和肝 Ｃｕ／ Ｚｎ Ｓ Ｏ Ｄ 活力均高于ｓｅｌｅｇｉｌｉｎｅ 组（ Ｐ＜ ００５） 。结论 Ｍ Ａ Ｏ Ｂ 抑制剂可提高衰老模型?
Objective The purpose of this study was to investigate the antioxidative mechanisms of monoamine oxidase B (MAO B) inhibitors in aging model mice induced by D galactose Methods The experimental mice were injected with D galactose 80 mg/Kg to produce a model of aging The effects of selegiline (0 25 mg·Kg -1 ) and 2 Hexyl N methyl propargylamine (2HxMP,10 mg·Kg -1 ) on the activities of Cu/Zn superoxide dismutase (Cu/ZnSOD), glutathine peroxidase (GSH Px), catalase and lipofuscin content in the brain and the liver were measured using chemiluminescence method, dithio bis 2 nitrobenzonic acid (DTNB) method, catalase detection kid and fluorescentmetric method, respectively Results In comparison with aging model group, the higher activities of Cu/ZnSOD in brain and liver and catalase in liver, but the lower content of lipofuscin in liver were observed both in 2HxMP group and in selegiline group; and the activities of GSH Px in brain and liver were also higher in 2HxMP group In addition, the Cu/ZnSOD level in liver and GSH Px in brain and liver were higher in 2HxMP group than in selegiline group Conclusions It is suggested that MAO B inhibitors could increase the activity of antioxidase and decrease the lipid peroxide level, which might play an important role in the prolongation of life span
出处
《中华精神科杂志》
CAS
CSCD
1999年第3期139-141,共3页
Chinese Journal of Psychiatry
关键词
单胺氧化酶
抑制剂
衰老
疾病模型
抗氯化作用
Monoamine oxidase inhibitors Aging Disease model, Animal Peroxidases Lipid peroxides
