摘要
100只13日龄白来航雏鸡随机分为5组,每组20只鸡,第1~4组为试验组,第5组为对照组,通过口腔灌服途径,给试验组鸡不同剂量的双氯芬酸（2.5 mg/kg、3.5 mg/kg、4.5 mg/kg、7.5 mg/kg体重）,喂药后24 h、48 h、72 h观察鸡的临床症状、病理组织学变化及临床病理学指标（血清尿酸（UA）、血清尿素氮（BUN）、血清K＋、Na＋、Ca2＋、Cl-和pH值等）检测,以阐明双氯芬酸的毒性作用。结果表明：给予7.5 mg/kg体重剂量的药物对雏鸡产生毒性作用,20只鸡中有4只鸡发生死亡,剖检发现有明显的内脏型痛风的病理变化,显微镜观察,肾小管内有尿酸盐沉积、肾小管坏死等病理变化,在肾、肝和脾脏中可见痛风石形成。在喂药后24 h,鸡血尿酸水平显著升高,BUN浓度也升高。其他试验组没有明显的变化。结论：双氯芬酸通过对肾脏的损伤作用,诱导鸡发生内脏型痛风。
One hundred,13-day-old,Leghorn chickens were randomly divided into 5 groups with 20 chickens each.In groups 1,2,3,4,5,birds received diclofenac sodium by oral feeding at the levels of 2.5 mg/kg,3.5 mg/kg,4.5mg/kg,7.5 mg/kg and 0 mg/kg respectively.24 h,48 h and 72 h post treatment,all birds were examined for clinical signs,necropsy findings,histopatological lesions and clinical pathological changes（Serum levels of uric acid（UA）,blood urea nitrogen（BUN）,potassium（K＋）,sodium（Na＋）,calcium（Ca2＋）,chloride（Cl-） and pH）to allow for the further characterization of diclofenac＇s mechanism of toxicity.The results showed that 4 dead birds of the 7.5 mg/kg-group developed gross lesions of visceral gout,while birds of other groups showed no manifesting visceral urate deposition.Microscopic examination revealed significant renal changes in dead birds manifesting visceral gout and these changes included urate deposits associated with tubular necrotic changes.24 h post treatment,serum level of uric acid and blood urea nitrogen level all increased in birds.It was concluded that development of gout in birds may be related to diclofenac impairing kidney.
出处
《中国兽医杂志》
CAS
北大核心
2011年第5期9-13,共5页
Chinese Journal of Veterinary Medicine
