摘要
对采自甘肃省3个冬虫夏草主产区域6个具有代表性地方的18个样本进行ISSR分析。15条ISSR引物共扩增得到条带清晰并呈多态性的95条谱带,每一引物扩增获得的ISSR条带数在3-9条之间,扩增片段集中在300-3,000bp。基于遗传相似性系数(GS)、不加权成对群算术平均法(UPGMA)构建的系统树分析,可以看出:分离自同一株虫草上不同部位的样本无显著遗传差异;同一地点样本间的遗传分化较小;不同地域的样本间存在着较大的遗传分化,遗传多样性较丰富。同时6个地方的冬虫夏草明显地分为3个区域,而在同一区域不同地方的样本又各聚为独立的一类,揭示了ISSR分子标记在分析冬虫夏草分布上表现出明显的地域性。
Inter-simple sequence repeats has been used in analyzing 18 Ophiocordyceps sinensis samples from 6 areas in Gansu Province.For 15 ISSR primers,each individual showed about 95 DNA bands which ranging in size from 300 to 3,000bp.Molecular phylogenetic tree constructed by genetic similarity coefficient （GS）,un-weighted pair group method with arithmetic meanscluster analysis （UPGMA） suggested that the samples isolated from different parts of the same caterpillar have no significant genetic differences;the genetic differentiation of the samples from the same location is not evident while that from different areas is comparatively evident.O.sinensis samples from 6 localities are obviously divided into 3 clustering groups and in the same clustering group there are independent local types.ISSR analysis reveals that O.sinensis shows its genetic variation correlated to its living enviroments.
出处
《菌物学报》
CAS
CSCD
北大核心
2011年第3期501-507,共7页
Mycosystema
关键词
冬虫夏草
ISSR
遗传分化
Ophiocordyceps sinensis
inter-simple sequence repeat （ISSR）
genetic differentiation
