摘要
观察对人血清y球蛋白热变性的抑制作用是筛选抗炎药物的一种体外实验方法。测定蛋白变性率的方法有两种,一种是测定加热后凝聚的蛋白,另一种是测定凝聚蛋白在醋酸中的不溶物。这两种方法在检验一般的抗炎药物时得到的结论基本上是一致的,但是在检验铜(Ⅱ)离子及其配合物时。两种方法得到相反的结论。本文旨在探讨铜(Ⅱ)离子在人血清r球蛋白热变性过程中,对形成外观凝聚物和醋酸不溶物的作用,并对两者的差异进行比较。
The effect of copper(Ⅱ) ion on the thermal denaturation of human serum gamma globulin in two different test systems was reported in this paper. It is showed in the results that copper(Ⅱ
)ion promoted the thermal coagulation of gamma globulin at 63℃, but inhibited the formation
of acetic acid insoluble aggregates at 90℃.
N-ethylmaleimide had a similar inhibitory effect as coppcr(II) ion in the latter test system. It is suggested that copper(Ⅱ) ion inhibit the cross-link between protein molecules through blocking sulfhydryl groups.
In the former test system, the thermal coagulation of gamma globulin did not involve the disulfide linkage between protein molecules because no acetic acid insoluble material appeared. The denaturation-promoting effect of copper(Ⅱ) ion was related to that the electrostatic distribution of protein molecules was changed by copper(Ⅱ) ion.
出处
《无机化学学报》
SCIE
CAS
CSCD
北大核心
1990年第1期109-111,共3页
Chinese Journal of Inorganic Chemistry
关键词
铜
球蛋白
热变性
The effect of copper(II) ion on the thermal denaturation of human serum gamma globulin in two different test systems was reported in this paper. It is showed in the results that copper(II)ion promoted the thermal coagulation of gamma globulin a
