摘要
目的 探讨子宫肉瘤组织的 Ｄ Ｎ Ａ 含量及细胞增殖水平与临床病理特征及预后的关系。方法 应用流式细胞术测定７５ 例石蜡包埋的子宫肉瘤组织中 Ｄ Ｎ Ａ 含量及细胞增殖水平。结果 晚期、低分化的子宫肉瘤患者，其 Ｄ Ｎ Ａ 异倍体率及 Ｄ Ｎ Ａ 指数（ Ｄ Ｉ） 均明显高于早期、高分化患者（ Ｐ＜ ０ ．０５） ；晚期患者肉瘤组织中 Ｓ期细胞比率（ Ｓ Ｐ Ｆ） 及增殖指数（ Ｐ Ｉ） 均明显高于早期患者（ Ｐ＜ ０ ．０５） 。 Ｄ Ｎ Ａ 异倍体患者和二倍体患者的５ 年生存率分别为２７ ％ 和７６ ％ ，两者比较，差异有极显著性（ Ｐ＜ ０ ．０１） ； Ｓ Ｐ Ｆ＞ ２０ ％ 患者和 Ｓ Ｐ Ｆ≤２０ ％ 患者的５ 年生存率分别为１０ ％ 和５６ ％ ，两者比较，差异有极显著性（ Ｐ＜ ０ ．０１） 。结论 Ｄ Ｎ Ａ 异倍体及 Ｓ Ｐ Ｆ、 Ｐ Ｉ值高与子宫肉瘤的恶性生物学行为有关， Ｄ Ｉ、 Ｓ Ｐ Ｆ、 Ｐ Ｉ和临床分期是影响预后的最重要因素。
Objective To investigate the relationship among DNA content, cell cycle phase analysis with the clinical pathological features and prognosis of uterine sarcomas. Methods DNA content and cell cycle phase analysis were performed on 75 paraffin embedded specimens of uterine sarcomas using flow cytometry. Results Both the aneuploidy rate and DNA index (DI) in specimens from advanced stage and poorly differentiated cases were significantly higher than those in specimens from early stage and well differentiated cases ( P <0.05). S phase fraction (SPF) and proliferation index (PI) were significantly higher in advanced stage than those in early stage( P <0.05). The 5 year suvival rates for DNA aneuploid and diploid patients were 27% and 76%, respectively ( P <0.01). The 5 year suvival rates for patients with a SPF>20% and those with SPF≤20% were 10% and 56% respectively ( P <0.01). Conclusion DI, SPF, PI and clinical stage are the most important factors affecting the prognosis.
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
1999年第9期551-554,共4页
Chinese Journal of Obstetrics and Gynecology
