摘要
目的:为评价聚合酶链反应(PCR)技术检测HBV DNA较之传统酶联免疫吸附测定法(ELISA)的优越性,评价HBsAg阴性消化科住院病人携带HBV的状态。方法:我们使用PCR技术检测了119例ELISA法检测HBsAg阴性的消化科住院病人的HBV DNA,并与103例ELISA法检测HBsAg阴性传染科住院肝炎病人相比较。结果:52.1%的消化科住院病人HBsAg、抗-HBs、抗-HBe、HBeAg、抗-HBe(统称HBVM)阳性,30.3％HBV DNA阳性;89.3％的传染料住院肝炎病人HBVM阳性,59.2％HBV DNA阳性。表明消化科住院病人的HBV感染率和HBV DNA阳性率均显著地低于传染科住院肝炎病人(P<0.005;P<0.005)。而且两组病人之间HBV DNA阳性率的差异仅存在于HBVM均阴性的HBV感染模式中。结论:PCR检测HBV DNA评价HBV携带状态更准确,弥补了ELISA检测HBsAg的不足。消化科住院病人中存在HBsAg阴性的HBV携带者,两组HBVM阳性病人实际上来自于同一群体。
Purpose: To evaluated whether the patients with negative HBsAg carry HBV in the depart-ment of gastroenterology of our hospital. Methods: The HBV DNA was measured with polymcrase chain reaction (PCR) technique in 119 patients with negative HBsAg in department of gastroenterology, and compared with 103 patients with negative HBsAg in department of. Results: The results showed that 52. 1% of the patients in the department of gastrogenterology were positive HBVM ( HBsAg, anti-HBs, HBeAg, ani-HBe and anti-HBc), and 30.3% HBV DNAwas positive in the patients. This two indexes were markedly lower compared with the patients in department of. The difference of the rate of positive HBV DNA in the two groups was only in the HBV infective model of negative HBVM. Conclusion: The results suggested that there were HBV carriers in negative HBsAg patients in the department of gastroen-teroloyg, and positive HBVM patients of the two groups actually come from the same group.
出处
《临床消化病杂志》
1999年第3期116-117,共2页
Chinese Journal of Clinical Gastroenterology
