摘要
目的:观察青蒿醇提物对H22肝癌小鼠的体内抑瘤作用。方法:取H22肝癌细胞接种于小鼠右腋皮下,随机分为模型对照组、5-氟尿嘧啶组(5-FU)及青蒿醇提物125 mg/(kg.d)组、500 mg/(kg.d)组,5-FU组肌肉注射给药,其余各组灌胃给药。连续给药8 d后,剖瘤称取质量,计算抑瘤率;利用高效液相色谱仪测定青蒿醇提物中青蒿素含量。结果:与模型对照组比较,青蒿醇提物各剂量组均不同程度的抑制肿瘤生长(P<0.01),青蒿醇提物125 mg/(kg.d)组和青蒿醇提物500 mg/(kg.d)组的抑瘤率分别为54.6%、50.6%;本实验青蒿醇提物中青蒿素含量为0.02%,而在青蒿素抗H22小鼠肝癌试验中,青蒿素在剂量为10 mg/(kg.d)时,对H22小鼠肝癌已没有明显的抑制作用。结论:青蒿醇提物对肝癌有明显的抑制作用;除青蒿素以外,中药青蒿中可能存在其它的抗肿瘤成分。
Objective ： To observe the inhibitory effect of Artemisia Ethanol Extract on tumor growth on murine transplanted hepatocarcinoma 22 （H：2） in vivo. Methods：Tumor inhibitory activities were tested in experimental tumor H22 mice. The tumor bearing mice were randomly divided into control group ,5-fluorouracil group （5-FU, 15 rag/（ kg .d）） and Artemisia Ethanol Extract treatment groups （125 rag/（ kg.d） group ,500 rag/（ kg. d） group）. These mice were given normal saline, or Artemisia Ethanol Extract different doses by gavage respectively once a day for 8 days, while 5-FU were given by intramuscular injection. Tumor growth state was observed and tumor inhibitory rate was calculated;To establish an HPLC method for the quantitative determination of the Artemisini in Artemisia Ethanol Extract. Resuhs：Artemisia Ethanol Extract treated groups with different doses could inhibit hepatocarcinoma 22（ H22 ） growth （P 〈 0.01 ） ,the inhibitory rate 125 mg/（ kg ~ d） group and 500 mg/（ kg ~ d） group respectively was 54.6% ,50.6% ;In the experiment of Artemisinin on tumor growth on murine transplanted hepatocarcinoma 22 （ H2z ）, It was showed that Artemisinin did not perform significant antitumor effects on hepatocarcinoma 22（ H22 ） at the dose of 10 rag/（ kg.d） ;The determination of the Artemisini in Artemisia Ethanol Extract was 0.02%. Conclusion：Artemisia Ethanol Extract can inhibit hepatocarcinoma growth;In addition to Anemisinin, other antitumor components may exist in Artemisia annua.
出处
《中医学报》
CAS
2011年第5期531-533,共3页
Acta Chinese Medicine
基金
国家中药材扶持项目(编号:广东/39)
关键词
青蒿醇提物
H22肝癌
抑瘤率
小鼠
artemisia ethanol extract
H22hepatocarcinoma
tumor inhibitory rate
mice
