摘要
用聚合酶链反应（ Ｐ Ｃ Ｒ）检测阿米巴肝脓肿患者血清标本中的溶组织内阿米巴 ３０ ０００ 蛋白基因，结果４２ 例患者中有３５ 例呈阳性反应，阳性率为８３．３％ ，低于脓标本 Ｐ Ｃ Ｒ阳性率（１００％ ）（ Ｐ＜ ０．０１）。３ 例细菌性肝脓肿、１ 例肝癌及１０ 例其它部位脓肿患者的血清和脓对照标本 Ｐ Ｃ Ｒ均呈阴性反应。对 Ｐ Ｃ Ｒ检测血标本诊断阿米巴肝脓肿的价值进行了讨论。
Polymerase chain reaction(PCR) was used to diagnose amebic liver abscess by detecting Entamoeba histolytica DNA in sera from patients with liver abscess. Oligonucleotide primers specific for gene encoding of the 30 000 molecule of Entamoeba histolytica were used in the test. Of 42 cases with amebic liver abscess as evidenced by typical clinical findings or very high titers of anti E. histolytica antibodies with ELISA, the E. histolytica DNAs in 35(83.3%) sera and 42(100%) liver aspirates were respectively found by PCR. Fourteen control samples(3 cases of bacterial liver abscess, 1 of liver cancer and 10 of abscess in other sites) were all negative to PCR. The results suggest that detecting the E. histolytica DNA in the serum with PCR was useful for diagnosing amebic liver abscess.
出处
《中国寄生虫病防治杂志》
CSCD
1999年第3期190-191,共2页
Chinese Journal of Parasitic Disease Control
