摘要
用 ５ 种不同的 Ｐ．ｖ．引物分别对间日疟原虫 Ｄ Ｎ Ａ 进行 Ｐ Ｃ Ｒ 扩增，在比较和分析各引物 Ｐ Ｃ Ｒ 扩增结果的敏感性和特异性的基础上，筛选出一对根据间日疟原虫线粒体细胞色素 Ｃ氧化酶基因序列设计的引物为最佳引物，其检测敏感度可达到每μｌ血中 ０．２４ 个疟原虫。同时还对血样本处理方法进行了改进，从而建立了一种更为简捷、快速、经济且适用于现场应用的间日疟原虫 Ｐ Ｃ Ｒ检测方法。并利用该方法对２３８ 例门诊发热病人滤纸干血滴样本进行检测，与传统镜检法进行了比较。结果显示， Ｐ Ｃ Ｒ 方法对间日疟的误诊率（０）和漏诊率（１．７％ ）明显比镜检的误诊率（４．２％ ）和漏诊率（４．２％ ）低，具有比常规镜检法更敏感、特异的优点。
Five pairs of different primers were compared for amplification of Plasmodium vivax in PCR. According to the sensitivity and specificity of PCR results, one pair of primers designed from Plasmodium vivax mitochondrial cytochrome C oxidase(CoⅢ) gene sequence was selected. PCR with the selected primers could detect 0.24 P. vivax parasite from 1 μl whole blood. The method for blood sample treatment was mordified and the PCR procedures were optimized so that it could be applicable in the field.In comparison with mocroscopic examination, 238 blood samples collected on filter paper from clinic fever cases in a mixed epidemic area in Yunnan Province were detected. The results showed that the false positive rate(0) and the false negative rate(1.7%) of PCR for P. vivax were both lower than those of microscopic examination(4.2% and 4.2% respectively). This confirmed that the established PCR was more sensitive and specific than microscopic examination for detection of P. vivax infection.
出处
《中国寄生虫病防治杂志》
CSCD
1999年第3期180-183,共4页
Chinese Journal of Parasitic Disease Control
