摘要
目的观察维生素C对链脲佐菌素(STZ)诱导糖尿病大鼠肾缺血-再灌注损伤的影响。方法 32只SD大鼠随机均分为四组:A、B、C组为糖尿病大鼠,B、C组行缺血-再灌注处理,A、B组每天腹腔注射生理盐水0.5 ml,C组注射维生素C 200 mg/kg(0.5 ml),D组为空白对照组。观察血糖、尿素氮(BUN)、肌酐(Cr)、丙二醛(MDA)、超氧化物歧化酶(SOD)、细胞色素C(Cytc)、半胱天冬酶-3(caspase-3)的变化。结果 B组BUN、Cr、MDA含量较C组明显升高(P<0.05),SOD的活性明显降低(P<0.05)。C组caspase-3和Cytc的表达明显减少,肾小管损伤、坏死显著减轻。结论维生素C能减轻STZ诱导糖尿病大鼠肾缺血-再灌注损伤的氧化应激,抑制细胞的凋亡及改善肾功能。
Objective To investigate the protective effects of ascorbic acid on renal ischemia/ reperfusion in STZqnduced diabetic rat. Methods Thirty-two SD rats were randomly divided into four groups with 8 rats each. The rats in group A, B, and C were induced diabetes by STZ,group B anct group c were exposed to isehemia-reperfusion. The rats in group A and group B were given norma sailne 0：5 ml/d i. P. for 15 days, group C were given ascorbie add 200 mg.kg-1 .d-3i.P. for 15 dfiys, ＇gf0Up D waS control. Results Compared with those in group B, the concentration of serum BUN,SCr and MDA were significant higher in group C（P〈0. 05）. The SOD level in group B was 0Diious I6wer than that in group C（P,〈O. 05）： In group C the tubular cell damage was lesser, the expressions of Cytc and caspase-3 were lower in group （3, Conclusion Vc treatment Can reduce renal injury and improve renal function by reducing lipid oxidation and cell apoptosis caused by IR in STZinduced diabetic rats.
出处
《临床麻醉学杂志》
CAS
CSCD
北大核心
2011年第5期504-506,共3页
Journal of Clinical Anesthesiology
关键词
抗坏血酸
糖尿病
再灌注损伤
Ascorbic acid
Diabetes mellitus
Reper{usion injury
