摘要
目的探讨血浆白三烯B4(LTB4)在过敏性紫癜(HSP)患儿免疫发病机制中的作用。方法 2009年5月-2010年3月河北医科大学第二医院儿科住院HSP患儿55例(HSP组),同时取健康同龄儿童20例作为健康对照组。采用ELISA法检测各组血浆LTB4水平,流式细胞术检测各组外周血CD4+ CD25+调节性T淋巴细胞及Th17细胞比例,同时观察LTB4水平与二者的相关性。应用SPSS 13.0软件进行统计学分析。结果 1.HSP患儿血浆LTB4[(63.5±20.8)ng.L-1]与健康对照组[(34.5±10.0)ng·L-1]比较明显升高(P<0.01);2.CD4+ CD25+调节性T淋巴细胞[(3.81±1.10)%]与健康对照组[(4.45±0.90)%]比较明显降低(P<0.05);3.Th17细胞[(1.80±0.66)%]与健康对照组[(0.52±0.24)%]比较明显升高(P<0.01);4.血浆LTB4水平与CD4+ CD25+调节性T淋巴细胞呈显著负相关(r=-0.67,P<0.05),与Th17细胞呈显著正相关(r=0.57,P<0.05)。结论 LTB4可能参与HSP患儿细胞免疫功能紊乱。
Objective To investigate the role of plasma leukotriene B4（LTB4）in immunological pathogenesis of Henoch-Schonlein purpura（HSP） in children.Methods Fifty-five children with HSP（HSP group） and 20 aged-matched healthy controls（healthy control group） were enrolled.Enzyme-linked immunosorbent assay was used to measure the level of plasma LTB4.Flow cytometric analysis was performed to detect the percentage of CD4＋CD25＋ regulation T cells and Th17 cells subpopulation.And the correlation between plasma LTB4 and frequency of CD4＋CD25＋ regulation T cells,Th17 cells were analyzed by SPSS 13.0 software.Results 1.The level of plasma LTB4 in children with HSP [（63.5±20.8） ng·L-1] was significantly higher than that in healthy control group [（34.5±10.0） ng·L-1].2.The frequency of CD4＋CD25＋ regulation T cells [（3.81±1.10） %] in HSP group was significantly lower than that in healthy control group[（4.45±0.90） %].3.The Th17 expression [（1.80±0.66） %]in HSP group was obviously higher than that in healthy control group[（0.52±0.24） %].4.The level of plasma LTB4 in children with HSP was strongly correlated with CD4＋CD25＋ regulation T cells（r=-0.67,P0.05） and Th17 cells（r=0.57,P0.05）.Conclusion The expression of plasma LTB4 maybe participate the immunogical dysfunction.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2011年第9期662-663,686,共3页
Journal of Applied Clinical Pediatrics
