摘要
目的:观察Ca2+/PKC通路在大鼠心肌细胞缺氧复氧状态下参与Egr-1高表达。方法:取生长5~6 d的大鼠心肌细胞分为对照(Control)组、缺氧复氧(H/R)组、溶剂(DMSO)组、EGTA组、维拉帕米(VER)组、H7组和BIM组。各组心肌细胞制作H/R模型。RT-PCR法检测培养心肌细胞中Egr-1 mRNA的表达水平。Western-blot法检测培养心肌细胞中Egr-1蛋白的表达水平。结果:与Control组相比,H/R组及DMSO组心肌细胞Egr-1 mRNA的表达水平明显增高(P<0.05);与H/R组相比,VER组、EGTA组、H7组、BIM组Egr-1 mRNA的表达明显下调,差异具有统计学意义(P<0.05)。与Control组相比,H/R组及DMSO组心肌细胞Egr-1蛋白表达水平明显增高(P<0.05);与H/R组相比,VER组、EGTA组、H7组、BIM组Egr-1蛋白的表达明显下调,差异具有统计学意义(P<0.05)。结论:在大鼠心肌细胞缺氧复氧状态下,Ca2+/PKC信号通路参与介导了Egr-1的高表达。
AIM： To study effects of Ca^2＋/PKC pathway on Egr-1 overexpression in hypox-ia/reoxygenation cardiomyocytes of rats. METHODS： Cultured cardiomyocytes of 5-6 day old rats were divided into control group, hypoxi a/reoxygenation （H/R） group, DMSO group,EGTA group , Verapamil BIM group. All groups group, of cardi H7 group and omyocytes derwent the treatment of H/R, and then un RT PCR and Western blot were used to test expres- sion of Egr-1 mRNA and Egr-1 protein respec-tively. RESULTS-There were high expression of Egr-1 mRNA in H/R group and DMSO group compared with control group （P〈0.05）. Vera-pamil, EGTA, H7 and Bim reduced the expres- sion of Egr-1 mRNA significantly compared with H/R group （P〈0.05）. There were high ex- pression of Egr-1 protein in H/R group and DM- SO group compared with control group （P〈0.05）. Verapamil, EGTA, H7 and Bim reduced the expression of Egr-1 protein significantly compared with H/R group （P〈0.05）. CON-CLUSION： Ca2t/PKC pathway plays a role on Egr-1 overexpression in hypoxia/reoxygenation cardiomyocytes of rats.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2011年第3期241-248,共8页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
国家自然科学基金-广东联合基金(U0932005)
中国高等教育博士点研究基金(200805600003)
