摘要
This study aimed to assess the inhibitory activities of methanol extracts from the microalgae Ankistrodesmus convolutus,Synechococcus elongatus,and Spirulina platensis against Epstein-Barr virus(EBV) in three Burkitt's lymphoma(BL) cell lines,namely Akata,B95-8,and P3HR-1.The antiviral activity was assessed by quantifying the cell-free EBV DNA using real-time polymerase chain reaction(PCR) technique.The methanol extracts from Ankistrodesmus convolutus and Synechococcus elongatus displayed low cytotoxicity and potent effect in re-ducing cell-free EBV DNA(EC50<0.01 μg/ml) with a high therapeutic index(>28 000).After fractionation by column chromatography,the fraction from Synechococcus elongatus(SEF1) reduced the cell-free EBV DNA most effectively(EC50=2.9 μg/ml,therapeutic index>69).Upon further fractionation by high performance liquid chromatography(HPLC),the sub-fraction SEF1'a was most active in reducing the cell-free EBV DNA(EC50=1.38 μg/ml,therapeutic index>14.5).This study suggests that microalgae could be a potential source of antiviral compounds that can be used against EBV.
This study aimed to assess the inhibitory activities of methanol extracts from the microalgae Ankistrodesmus convolutus, Synechococcus elongatus, and Spirulina platensis against Epstein-Barr virus （EBV） in three Burkitt＇s lymphoma （BL） cell lines, namely Akata, B95-8, and P3HR-1. The antiviral activity was assessed by quantifying the cell-free EBV DNA using real-time polymerase chain reaction （PCR） technique. The methanol extracts from Ankistrodesmus convolutus and Synechococcus elongatus displayed low cytotoxicity and potent effect in re- ducing cell-free EBV DNA （EC50〈0.01 μg/ml） with a high therapeutic index （〉28000）. After fractionation by column chromatography, the fraction from Synechococcus elongatus （SEF1） reduced the cell-free EBV DNA most effectively （EC50=2.9 μg/ml, therapeutic index〉69）. Upon further fractionation by high performance liquid chromatography （HPLC）, the sub-fraction SEFI＇a was most active in reducing the cell-free EBV DNA （EC50=1.38 μg/ml, therapeutic index〉14.5）. This study suggests that microalgae could be a potential source of antiviral compounds that can be used against EBV.
基金
Project (IRPA:06-05-01-0000-PR0054105-04) supported by the Ministry of Science,Technology,and Innovation (MOSTI) of Malaysia
