摘要
目的硼酸盐生物玻璃具有较高的化学反应活性,可以诱导骨再生。通过调整配方构建新型硼酸盐生物玻璃,探讨其对体外培养成骨细胞行为的影响,为其下一步的体内研究及临床应用奠定实验基础。方法采用熔融法制备Na2O-K2O-MgO-CaO-P2O5-B2O3-SrO新型硼酸盐生物玻璃。并根据ISO10993-12:2007的要求制备材料初次浸提液及二次浸提液。取体外培养第5～15代MC3T3-E1细胞,分别与初次浸提液、二次浸提液共培养,以α-MEM培养基为对照。观察新型硼酸盐生物玻璃对成骨细胞增殖率、蛋白合成、ALP活性、细胞凋亡以及细胞横向、纵向迁移的影响。结果初次浸提液组、二次浸提液组与对照组成骨细胞增殖吸光度A值分别为0.356 0±0.018 7、0.331 0±0.025 4、0.204 0±0.013 8,各组间比较差异均有统计学意义(P<0.05)。初次浸提液组、二次浸提液组与对照组总蛋白含量分别为(382.847±9.521)、(226.071±5.847)、(220.248±8.213)U,初次浸提液组与对照组、二次浸提液组比较,差异均有统计学意义(P<0.05),但二次浸提液组与对照组比较,差异无统计学意义(P>0.05)。初次浸提液组、二次浸提液组及对照组ALP活性分别为(0.013 01±0.000 39)、(0.012 93±0.000 44)、(0.012 92±0.000 35)U/mg;细胞凋亡率分别为7.03%±1.95%、6.46%±2.88%、6.18%±2.21%;细胞横向迁移距离分别为(137.50±11.43)、(134.98±10.50)、(135.21±8.66)μm;Transwell中穿膜细胞数分别为(10.92±4.99)、(10.07±2.50)、(9.81±2.64)个/视野;以上指标3组间两两比较差异均无统计学意义(P>0.05)。结论新型硼酸盐生物玻璃具有良好的成骨细胞相容性,对细胞增殖、分泌、迁移等行为均有一定调节作用。
Objective As a bioactive material,the osteogenic activity of borate bioglass has been proved.To design a novel borate bioglass according to an improved formula and to investigate the effects of the borate bioglass on osteoblasts in vitro for further research and potential clinical application.Methods The novel Na2O-K2O-MgO-CaO-P2O5-B2O3-SrO borate bioglass was prepared by melting process.The initial and secondary extracts were prepared according to ISO10993-12：2007 respectively with different extract time of 0-24 hours and 24-48 hours.The osteoblasts（MC3T3-E1） of the 5th-15th passages from mouse were cocultured with the initial（initial extract group） and secondary（secondary extract group） extracts,respectively,to assess the effects of the borate bioglass on the cell proliferation,protein synthesis,alkaline phosphatase（ALP） activity,cell apoptosis,and cell migration;while α-MEM medium without addition of extract served as control group.Results The absorbance values at 450 nm were 0.356 0±0.018 7,0.331 0±0.025 4,and 0.204 0±0.013 8 in initial extract,secondary extract,and control groups,respectively,showing significant differences among 3 groups（P0.05）.The total protein contents were（382.847±9.521）,（226.071±5.847）,and（220.248±8.213） U in initial extract,secondary extract,and control groups,respectively;there were significant differences between initial extract group and control group,and between initial extract group and secondary group（P0.05）,but there was no significant difference between secondary extract group and control group（P0.05）.However,no significant difference was observed in the ALP activity [（0.013 01±0.000 39）,（0.012 93±0.000 44）,and（0.012 92±0.000 35） U/ mg],apoptosis rate（7.03%±1.95%,6.46%±2.88%,and 6.18%±2.21%）,horizontal migration [（137.50±11.43）,（134.98±10.50）,（135.21±8.66） μm],and transmembrane cell number [（10.92±4.99）,（10.07±2.50）,and（9.81±2.64） cells/ field] among initial extract,secondary extract,and control groups（P0.05）.Conclusion This novel borate bioglass has excellent cytocompatibility,which plays regulatory effects on the cell proliferation,secretion,and migration.
出处
《中国修复重建外科杂志》
CAS
CSCD
北大核心
2011年第5期606-609,共4页
Chinese Journal of Reparative and Reconstructive Surgery
基金
上海市科学技术委员会纳米专项支持项目(0952nm04300)~~
