摘要
目的探讨乌司他丁对白介素-1β(interleukin-1,βIL-1β)诱导的兔椎间盘髓核细胞中诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)、基质金属蛋白酶2(matrix metalloproteinase 2,MMP-2)和MMP-3表达的影响。方法用酶消化法提取兔椎间盘髓核细胞并进行体外培养。将兔髓核细胞随机分为6组:正常对照组、乌司他丁对照组、IL诱导组和3个不同浓度乌司他丁抑制组。作用48 h后用分光光度法检测各组培养液中iNOS活性,并用细胞免疫组化法检测MMP-2、MMP-3的表达变化。结果各抑制组中iNOS含量均低于诱导组,以320单位/毫升抑制组最显著,差异有统计学意义(P<0.01);各抑制组中MMP-2、MMP-3的表达均低于诱导组,差异有统计学意义(P<0.01),且其表达降低的程度与药物的浓度呈正比。结论体外条件下,一定浓度的乌司他丁能够在一定程度上抑制IL-1β诱导的椎间盘细胞中iNOS、MMP-2及MMP-3的表达,减少对蛋白多糖及胶原的降解。
Objective To observe the effect of ulinastatin on expression of inducible nitric oxide synthase（iNOS）,matrix metalloproteinase 2（MMP-2） and MMP-3 in degenerative nucleus pulposus cells of rabbit induced by interleukin-1β（IL-1β）.Methods Nucleus pulposus cells of rabbit collected and cultured by enzyme digestion in vitro were divided into 6 groups randomly： normal control group,ulinastatin control group,IL induced group and 3 inhibited gourps with ulinastatin at different concentration.After 48 h of intervention,nucleus pulposus cells were collected for detection of the content of iNOS with spectrophotometric method and the expression of MMP-2、MMP-3 by immunohistochemistry staining.Results Compared with the IL induced group,the content of iNOS in each ulinastatin inhibited group was decreased,most significantly in the 320 U/mL inhibited group,the differences were statistically significant（P0.01）;the expression of MMP-2,MMP-3 proteins in nucleus pulposus cells were decreased in the ulinastatin inhibited groups,the difference was statistically significant（P0.01）,and the decrease was in inverse proportion to ulinastatin concentration.Conclusion Ulinastatin in a certain concentration can inhibit the increased expression of MMP-2,MMP-3 and iNOS effectively and reduce the degradation of proteoglycan and collagen in nucleus pulposus cells induced by IL-1β in vitro.
出处
《脊柱外科杂志》
2011年第1期46-50,共5页
Journal of Spinal Surgery
关键词
兔
椎间盘
胰蛋白酶抑制剂
基质金属蛋白酶2
基质金属蛋白酶3
一氧化氮合酶
Rabbits
Intervertebral disk
Trypsin inhibitors
Matrix metalloproteinase 2
Matrix metalloproteinase 3
Nitric oxide synthase
