摘要
目的：研究镇冲降逆止呕方对家鸽呕吐模型的防治作用及对神经递质（多巴胺和5-羟色胺）的影响。方法：分别以口服硫酸铜和静脉注射顺铂复制家鸽呕吐模型，观察记录呕吐潜伏期和呕吐频率；以荧光分光光度计检测样品荧光强度，计算脑组织中DA和5-HT的含量。结果：灌胃给予镇冲降逆止呕方0．5mL／100g、1mL／100g 10d后，能延长家鸽呕吐模型的呕吐潜伏期，减少呕吐频率；镇冲降逆止呕方灌胃给药后能在一定程度上降低蒙鸽呕吐模型脑组织中DA和5-HT含量。结论：镇冲降逆止呕方时家鸽呕吐模型有防治作用。
Objective：To study the effect of Zhen--chong--jiang--ni--zhi--ou--fang on the pigeon vomiting model and the content of dopamine and 5--hydroxytryptamine. Methods：Adopting the pigeon vomiting model induced copper sulfate and DDP to observe the vomiting delitescence and frequency; the contents of dopamine and 5- hydroxytryptamine in brain tissue were calculated accoding to the fluorescence intensity detected hy fluo- rometer. Results：The pigeons were given Zhen--chong--jiang--ni--zhi--ou--fang（0.5mL/100g,1mL/100g daily,ig. ） for 10 days. Zhen--chong-- jiang--ni-zhi-ou--fang can reduce the vomiting delitescence,frequency and the contents of dopamine and 5- hydroxytryptamine in brain tissue. Conclusion： Zhen--chong--jiang--ni--zhi-ou--fang has preventive and therapeutic effects on the pigeon vomiting model.
基金
基金项目：四川省泸州市科技局资助
关键词
镇冲降逆止呕方
呕吐
多巴胺
5-羟色胺
Zhen-- chong--jiang -- ni-- zhi-- ou-- fang
vomit
dopamine
5 -- hydroxytryptamine
