摘要
目的:观察制萎扶胃浓缩丸(FW)对慢性萎缩性胃炎(CAG)模型大鼠胃分泌功能和胃黏膜组织形态的影响,探讨FW防治CAG的作用机制。方法:采用综合因素(30%乙醇与2%水杨酸钠混合液与10 mmol.L-1去氧胆酸钠交替ig,0.05%氨水日常饮用,结合主动免疫法,并令其饥饱失常。)诱发建立大鼠CAG模型,设FW 1.67,0.84.0.42 g·kg-1组,阳性药麻罗丹1.26 g·kg-1组,ig,75 d后比色法测定胃蛋白酶活性,测定胃液分泌量、胃酸pH、胃液总酸度并进行胃黏膜组织形态学观察。结果:FW明显改善CAG模型大鼠一般状态,高剂量促进胃液分泌、提高胃液总酸度(P<0.05);各剂量均能增加胃蛋白酶活性(P<0.01),效应与剂量呈正相关;对CAG模型大鼠胃黏膜病理形态具有较好的改善作用。结论:FW能改善CAG模型大鼠胃分泌功能,改善慢性萎缩性胃炎胃黏膜组织形态。
Objective：To observe the effect of Zhiwei Fuwei concentrated pill（FW）on gastric secretory function and histomorphology of gastric mucosa in chronic atrophic gastritis（CAG） model rats,and to investigate the mechanism of FW in prevention and treatment of CAG.Method：An experimental CAG model was established by comprehensive factors（including alternative ingestion with 30% ethanol and 2% sodium salicylate mixed solution and 10 mmol·L-1 sodium deoxycholate,0.05% ammonia by drinking daily,combining with active immunization method,followed by either hunger or gorge）in Wistar rats.Three doses of FW were used（1.67,0.84,0.42 g·kg-1 ig,35 d）as treatment.The pepsin activity was tested by colorimetry,the content of gastric secretion,gastric acid pH and total acid of gastric were determined,the histomorphology of gastric mucosa was observed.Result：FW could improve the general conditions significantly for CAG rats.The high dosage of FW could increase gastric secretion and total acid of gastric（P0.05）.All dosage of FW could promote pepsin activity（P0.01）.There was a positive correlation between the effect and dosage.It had good improving effect on pathologic morphology of gastric mucosa.Conclusion：FW can improve gastric secretory function and histomorphology of gastric mucosa in CAG model rats.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第10期173-176,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
甘肃省卫生厅重点中医药科研项目(GZK-2009-19)
