摘要
目的建立同时测定消毒剂中9种酚类化合物的高效液相色谱法。方法在C18(4.6mm×250mm,5μm)色谱柱上,流动相为甲醇-0.22%庚烷磺酸钠水溶液(pH3.5)梯度洗脱,流速为1.0 ml/min,检测波长为280nm,柱温为25℃的条件下检测。结果该方法中各组分的线性范围为5～200mg/L,r>0.9995,检出限为1mg/L,相对标准偏差小于4.8%,加标回收率在97.6%～108.2%之间。结论该方法操作简便、快速、灵敏度高,可为消毒剂的常规分析和有效成分的质量控制提供参考。
Objective A high performance liquid chromatography method was established for determination of 9 phenolic compounds in the disinfectant.Methods on a C18（4.6mm×250mm,5μm） chromatography column,use the methol-0.22% sodium heptane solution（pH3.5） as mobile phase ladder washing,under the following conditions： flow rate 1.0ml/min,detection wave 280nm,and the column temperature 25℃.Results In this method,the linear range of each compound was 5-200mg/L and the correlation coefficients was more than 0.9995.The detection limit was 1mg/L.The RSD was less than 4.8% and standard addition recovery varied from 97.6% to 108.2%.Conclusion This method was easy to operate,fast and with high sensitivity.It was suitable for routine analysis for disinfectant and can be used for the quality control of effective compounds in disinfectant.
出处
《卫生研究》
CAS
CSCD
北大核心
2011年第3期379-381,共3页
Journal of Hygiene Research
