摘要
目的:研究C反应蛋白(C-reactive protein,CRP)对小鼠主动脉内皮细胞胞内游离钙[Ca2+]i的作用。方法:原代培养小鼠主动脉内皮细胞,取生长状态良好的内皮细胞分为对照组(未加CRP)和不同浓度CRP处理组,25 min后观察各组内皮细胞[Ca2+]i。将内皮细胞用1μmol/L阿托伐他汀预处理30 min后,加入100 mg/L CRP,观察阿托伐他汀对CRP作用的影响。结果:与对照组相比,CRP可显著升高内皮细胞胞内[Ca2+]i并呈剂量依赖关系;阿托伐他汀可明显降低CRP引起的[Ca2+]i升高。结论:CRP可导致和加重小鼠主动脉内皮细胞的炎症反应,阿托伐他汀可部分拮抗CRP的致炎作用。
Objective：To investigate the effect of CRP on the i in mice aorta endothelial cells（ECs）.Methods：Mice aortic endothelial cells were isolated and cultured.ECs were divided into control group and CRP treated group,and the changes of i was examined after 25 min.For the effect of atorvastatin,the cells were pre-incubated 30 min by atorvastatin（1 μmol/L）prior to adding CRP（100 mg/L）.Results：i of ECs significantly increased after the treatment of CRP in a dose dependent manner.Furthermore,atorvastatin partially abrogated the up-regulation of i.Conclusion：Inflammatory of ECs was up-regulation in response to CRP stimulation.Meanwhile,the research also demonstrated that atorvastatin partial suppressed inflammatory induced by CRP treatment.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2011年第5期679-683,共5页
Journal of Nanjing Medical University(Natural Sciences)
基金
国家自然科学基金资助项目(30770894)
