芸薹属基本种45S rDNA重复序列的染色体定位被引量：1

Chromosomal localization of 45S rDNA in Brassica diploids

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摘要用45S rDNA作探针,对芸薹属3个二倍体种(白菜型油菜、甘蓝和黑芥)进行有丝分裂和减数分裂的原位杂交分析,发现在白菜型油菜的20条染色体中有10条、黑芥16条染色体中有6条、甘蓝18条染色体中有4条染色体上存在杂交信号。其中白菜型油菜和黑芥的减数分裂为首次报道,研究结果对研究芸薹属二倍体基因组A、B或C的亲缘关系以及基因组间的相互进化关系有一定的意义。 The position and number of 45S rDNA on mitotic and meiotic chromosomes of the three basic diploid species （Brassica rapa, 13. oleracea and I3. nigra） were detected by fluorescence in situ hybridization （FISH） analysis using 45S rDNA as probe. Ten out of 20 chromosomes can be easily identified in B. rapa, six out of 16 in B. nigra, and four out of 18 in B. olerucea. Among them, meiotic FISH analysis of 45S rDNA of B. rapa and B. nigra was reported for the first time. These investigations are useful for identifying the chromosomes of genome A, t3 and C and studying the evolution and phylogeny of the Brassica genomes.

作者张永泰孔芳李爱民王幼平

机构地区江苏里下河地区农业科学研究所安徽工程大学生物与化学工程学院扬州大学生物科学与技术学院

出处《扬州大学学报（农业与生命科学版）》 CAS CSCD 北大核心 2011年第1期10-13,F0003,共5页 Journal of Yangzhou University：Agricultural and Life Science Edition

基金国家自然科学基金资助项目(30671166)

关键词芸薹属重复序列原位杂交染色体定位 Brassica repeat sequence FISH chromosomal localization

分类号 Q813.4 [生物学—生物工程]

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